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A Simple and Rapid UPLC-PDA Method for Quality Control of Nardostachys jatamansi
- Source :
- Planta Medica. 84:536-543
- Publication Year :
- 2017
- Publisher :
- Georg Thieme Verlag KG, 2017.
-
Abstract
- Nardostachys jatamansi is a well-documented herbal agent used to treat digestive and neuropsychiatric disorders in oriental medicinal systems. However, few simple, rapid, and comprehensive methods were reported for quality assessment and control of N. jatamansi. Herein, a UPLC with photodiode array detection method was developed for both fingerprint investigation of N. jatamansi and simultaneous quantitative analysis of the six serotonin transporter modulatory constituents in N. jatamansi. For chromatographic fingerprinting, 24 common peaks were selected as characteristic peaks to assess the consistency of N. jatamansi samples from different retail sources. Six of the common peaks (5, 7, 12, and 16 – 18) were identified as desoxo-narchinol A, buddleoside, isonardosinone, nardosinone, kanshone H, and (−)-aristolone, respectively, by phytochemical investigation. Five of the six compounds significantly either enhanced or inhibited serotonin transporter activity, while (−)-aristolone (18) didnʼt show any serotonin transporter activity. In quantitative analysis, the six compounds showed good linearity (r > 0.999) within test ranges. The precision, expressed as relative standard deviation, was in the range of 0.25 – 2.77%, and the recovery of the method was in the range of 92 – 105%. The UPLC-photodiode array detection-based fingerprint analysis and quantitative methods reported here could be used for routine quality control of N. jatamansi.
- Subjects :
- Quality Control
Uplc pda
Pharmaceutical Science
01 natural sciences
High-performance liquid chromatography
Nardostachys
Analytical Chemistry
chemistry.chemical_compound
Drug Discovery
Chromatography, High Pressure Liquid
Serotonin transporter
Pharmacology
Chromatography
biology
010405 organic chemistry
Chemistry
010401 analytical chemistry
Organic Chemistry
Nardostachys jatamansi
biology.organism_classification
0104 chemical sciences
Complementary and alternative medicine
Phytochemical
biology.protein
Molecular Medicine
Nardosinone
Quantitative analysis (chemistry)
Drugs, Chinese Herbal
Subjects
Details
- ISSN :
- 14390221 and 00320943
- Volume :
- 84
- Database :
- OpenAIRE
- Journal :
- Planta Medica
- Accession number :
- edsair.doi.dedup.....033b6188ae101a2869e5888111235988
- Full Text :
- https://doi.org/10.1055/s-0043-123655