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A Simple and Rapid UPLC-PDA Method for Quality Control of Nardostachys jatamansi

Authors :
Hong-Hua Wu
Meng Wang
Yan Zhu
Xiumei Gao
Miaomiao Jiang
Tian-Xiang Li
Yun Seon Song
Guo Nan
Yan-Tong Xu
Jiaming Wang
Weize Zhang
Source :
Planta Medica. 84:536-543
Publication Year :
2017
Publisher :
Georg Thieme Verlag KG, 2017.

Abstract

Nardostachys jatamansi is a well-documented herbal agent used to treat digestive and neuropsychiatric disorders in oriental medicinal systems. However, few simple, rapid, and comprehensive methods were reported for quality assessment and control of N. jatamansi. Herein, a UPLC with photodiode array detection method was developed for both fingerprint investigation of N. jatamansi and simultaneous quantitative analysis of the six serotonin transporter modulatory constituents in N. jatamansi. For chromatographic fingerprinting, 24 common peaks were selected as characteristic peaks to assess the consistency of N. jatamansi samples from different retail sources. Six of the common peaks (5, 7, 12, and 16 – 18) were identified as desoxo-narchinol A, buddleoside, isonardosinone, nardosinone, kanshone H, and (−)-aristolone, respectively, by phytochemical investigation. Five of the six compounds significantly either enhanced or inhibited serotonin transporter activity, while (−)-aristolone (18) didnʼt show any serotonin transporter activity. In quantitative analysis, the six compounds showed good linearity (r > 0.999) within test ranges. The precision, expressed as relative standard deviation, was in the range of 0.25 – 2.77%, and the recovery of the method was in the range of 92 – 105%. The UPLC-photodiode array detection-based fingerprint analysis and quantitative methods reported here could be used for routine quality control of N. jatamansi.

Details

ISSN :
14390221 and 00320943
Volume :
84
Database :
OpenAIRE
Journal :
Planta Medica
Accession number :
edsair.doi.dedup.....033b6188ae101a2869e5888111235988
Full Text :
https://doi.org/10.1055/s-0043-123655