Back to Search
Start Over
Genes responsive to rapamycin and serum deprivation are clustered on chromosomes and undergo reorganization within local chromatin environments
- Source :
- Biochemistry and cell biology = Biochimie et biologie cellulaire. 98(2)
- Publication Year :
- 2019
-
Abstract
- We previously demonstrated that genome reorganization, through chromosome territory repositioning, occurs concurrently with significant changes in gene expression in normal primary human fibroblasts treated with the drug rapamycin, or stimulated into quiescence. Although these events occurred concomitantly, it is unclear how specific changes in gene expression relate to reorganization of the genome at higher resolution. We used computational analyses, genome organization assays, and microscopy, to investigate the relationship between chromosome territory positioning and gene expression. We determined that despite relocation of chromosome territories, there was no substantial bias in the proportion of genes changing expression on any one chromosome, including chromosomes 10 and 18. Computational analyses identified that clusters of serum deprivation and rapamycin-responsive genes along the linear extent of chromosomes. Chromosome conformation capture (3C) analysis demonstrated the strengthening or loss of specific long-range chromatin interactions in response to rapamycin and quiescence induction, including a cluster of genes containing Interleukin-8 and several chemokine genes on chromosome 4. We further observed that the LIF gene, which is highly induced upon rapamycin treatment, strengthened interactions with up- and down-stream intergenic regions. Our findings indicate that the repositioning of chromosome territories in response to cell stimuli, this does not reflect gene expression changes occurring within physically clustered groups of genes.
- Subjects :
- Serum
Chromosomes, Artificial, Bacterial
Biology
Biochemistry
Genome
Chromosome Painting
03 medical and health sciences
0302 clinical medicine
Gene expression
medicine
Cluster Analysis
Humans
Serum deprivation
Fibroblast
Molecular Biology
Gene
In Situ Hybridization, Fluorescence
030304 developmental biology
Cell Proliferation
Gene Library
Cell Nucleus
Sirolimus
0303 health sciences
Chromosomes, Human, Pair 10
Genome, Human
Gene Expression Profiling
Interleukin-8
Computational Biology
Cell Biology
Fibroblasts
Chromatin
Cell biology
medicine.anatomical_structure
Gene Expression Regulation
030220 oncology & carcinogenesis
Chromosome Territory
Multigene Family
Chromosomes, Human, Pair 18
Subjects
Details
- ISSN :
- 12086002
- Volume :
- 98
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Biochemistry and cell biology = Biochimie et biologie cellulaire
- Accession number :
- edsair.doi.dedup.....0332b3d512276cf21bdefc5a1115d39f