Back to Search Start Over

Additional file 1 of CATI: an efficient gene integration method for rodent and primate embryos by MMEJ suppression

Authors :
Chen, Hongyu
Liu, Xingchen
Li, Lanxin
Tan, Qingtong
Li, Shiyan
Li, Li
Li, Chunyang
Fu, Jiqiang
Lu, Yong
Wang, Yan
Sun, Yidi
Luo, Zhen-Ge
Lu, Zongyang
Sun, Qiang
Liu, Zhen
Publication Year :
2023
Publisher :
figshare, 2023.

Abstract

Additional file 1: Fig. S1. Evaluation of editing efficiency, NHEJ/MMEJ ratio, DNA repair-related gene expression, and knockdown approaches in embryos. Fig. S2. Integration analysis of Cre knock-in at Lypd1 and Calcr loci. Fig. S3. Two-cell strategy test in mouse and monkey embryos. Fig. S4. CATI application in nucleotide replacement of monkey CDKL5 locus and GOTI experiment for off-target analysis.

Details

Database :
OpenAIRE
Accession number :
edsair.doi.dedup.....031851456e9b050151d30abaf6009b00
Full Text :
https://doi.org/10.6084/m9.figshare.23573711.v1