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Restoration of polar-flagellum motility and biofilm-forming capacity in the mmsB1 mutant of the alphaproteobacterium Azospirillum brasilense Sp245 points to a new role for a homologue of 3-hydroxyisobutyrate dehydrogenase
- Source :
- Canadian Journal of Microbiology. 65:144-154
- Publication Year :
- 2019
- Publisher :
- Canadian Science Publishing, 2019.
-
Abstract
- The bacterium Azospirillum brasilense can swim and swarm owing to the rotation of a constitutive polar flagellum (Fla) and inducible lateral flagella, respectively. They also form biofilms on various interfaces. Experimental data on flagellar assembly and social behaviours in these bacteria are scarce. Here, for the first time, the chromosomal coding sequence mmsB1 for a homologue of 3-hydroxyisobutyrate dehydrogenase (protein accession Nos. ADT80774 and E7CWE2) was shown to play a role in the assembly of motile Fla and in biofilm biomass accumulation. In the previously obtained mutant SK039 of A. brasilense Sp245, an Omegon-Km insertion in mmsB1 was concurrent with changes in cell-surface properties and with suppression of Fla assembly (partial) and Fla-dependent motility (complete). Here, the immotile leaky Fla− mutant SK039 was complemented with the expression vector pRK415-borne mmsB1 gene of Sp245. In the complemented mutant, the elevated relative cell hydrophobicity and changed relative membrane fluidity of SK039 returned to the wild-type levels; also, biofilm biomass accumulation increased and even reached Sp245’s levels under nutritionally rich conditions. In strain SK039 (pRK415-mmsB1), the percentage of cells with Fla became significantly higher than that in mutant SK039, and the Fla-driven swimming velocity was equal to that in strain Sp245.
- Subjects :
- Immunology
Mutant
Azospirillum brasilense
Flagellum
Applied Microbiology and Biotechnology
Microbiology
03 medical and health sciences
Genetics
3-hydroxyisobutyrate dehydrogenase
Molecular Biology
030304 developmental biology
0303 health sciences
biology
030306 microbiology
Chemistry
Biofilm
General Medicine
biology.organism_classification
Cell biology
Alcohol Oxidoreductases
Flagella
Biofilms
Polar
Hydrophobic and Hydrophilic Interactions
Flagellum motility
Bacteria
Subjects
Details
- ISSN :
- 14803275 and 00084166
- Volume :
- 65
- Database :
- OpenAIRE
- Journal :
- Canadian Journal of Microbiology
- Accession number :
- edsair.doi.dedup.....0304fce554c7fd9a30a97690fd0e6575