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Antileishmanial activity and immunomodulatory effect of secosubamolide, a butanolide isolated from Nectandra oppositifolia (Lauraceae)
- Source :
- Journal of Venomous Animals and Toxins including Tropical Diseases, Volume: 25, Article number: e20190008, Published: 19 AUG 2019, Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 25, Journal of Venomous Animals and Toxins including Tropical Diseases v.25 2019, The Journal of venomous animals and toxins including tropical diseases, Universidade Estadual Paulista (UNESP), instacron:UNESP
- Publication Year :
- 2019
- Publisher :
- Centro de Estudos de Venenos e Animais Peçonhentos, 2019.
-
Abstract
- Background: Visceral leishmaniasis is a complex neglected tropical disease caused by Leishmania donovani complex. Its current treatment reveals strong limitations, especially high toxicity. In this context, natural products are important sources of new drug alternatives for VL therapy. Therefore, the antileishmanial and immunomodulatory activity of compounds isolated from Nectandra oppositifolia (Lauraceae) was investigated herein. Methods: The n-hexane extract from twigs of N. oppositifolia were subjected to HPLC/HRESIMS and bioactivity-guided fractionation to afford compounds 1 and 2 which were evaluated in vitro against Leishmania (L.) infantum chagasi and NCTC cells. Results: The n-hexane extract displayed activity against L. (L.) infantum chagasi and afforded isolinderanolide E (1) and secosubamolide A (2), which were effective against L. (L.) infantum chagasi promastigotes, with IC50 values of 57.9 and 24.9 µM, respectively. Compound 2 was effective against amastigotes (IC50 = 10.5 µM) and displayed moderate mammalian cytotoxicity (CC50 = 42 µM). The immunomodulatory studies of compound 2 suggested an anti-inflammatory activity, with suppression of IL-6, IL-10, TNF with lack of nitric oxide. Conclusion: This study showed the antileishmanial activity of compounds 1 and 2 isolated from N. oppositifolia. Furthermore, compound 2 demonstrated an antileishmanial activity towards amastigotes associated to an immunomodulatory effect.
- Subjects :
- lcsh:Arctic medicine. Tropical medicine
Immunomodulatory
lcsh:RC955-962
Nectandra oppositifolia
Context (language use)
Toxicology
lcsh:RA1190-1270
lcsh:Zoology
parasitic diseases
medicine
lcsh:QL1-991
Antileishmanial
Cytotoxicity
Amastigote
IC50
lcsh:Toxicology. Poisons
biology
Traditional medicine
Secosubamolide
Chemistry
Leishmania (L.) infantum chagasi
Lauraceae
medicine.disease
biology.organism_classification
Leishmania
In vitro
Infectious Diseases
Visceral leishmaniasis
Animal Science and Zoology
Parasitology
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Journal of Venomous Animals and Toxins including Tropical Diseases, Volume: 25, Article number: e20190008, Published: 19 AUG 2019, Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 25, Journal of Venomous Animals and Toxins including Tropical Diseases v.25 2019, The Journal of venomous animals and toxins including tropical diseases, Universidade Estadual Paulista (UNESP), instacron:UNESP
- Accession number :
- edsair.doi.dedup.....02f461a5369ab7548eeea19ccc3f2bc2