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Easy and efficient chemical sterilization of the culture medium for in vitro growth of gerbera using chlorine dioxide (ClO2)
- Source :
- Ornamental Horticulture, Vol 24, Iss 3, Pp 218-224 (2018)
- Publication Year :
- 2018
- Publisher :
- Sociedade Brasileira de Floricultura e Plantas Ornamentais, 2018.
-
Abstract
- Micropropagation techniques changed the production of clonal plantlets in the world. However, the high costs of micropropagated plantlets continue as the main constraint for the expansion of the technique. This paper aimed to test the use of the chemical sterilization of culture medium using chlorine dioxide (ClO2) for in vitro cultivation of gerbera. There was used gerbera in vitro shoots in the stage of rooting for these experiments, using 0.0035%, 0.0070% and 0.0105% of chlorine dioxide in the culture medium. Also, peracetic acid was tested previously for sterilization, but resulted in microbial contamination. Chemical sterilization of the culture medium was successfully using ClO2 at 0.0035% to 0.0105% (100% decontamination) at rooting and elongation stage of gerbera with production of plantlets with similar (number of leaves, total and root fresh weight) or higher quality (mainly aerial part) at rooting/elongation stage, compared with autoclaved culture medium. The increase of concentration of ClO2 also resulted in increasing of height and fresh weight of aerial part of gerberas. The ClO2 could replace the autoclaving with production of sterilized culture medium without phytotoxic problems to gerbera in vitro cultivation.
- Subjects :
- 0106 biological sciences
0301 basic medicine
Gerbera
Chlorine dioxide
biology
Gerbera jamesonii, micropropagation, sterilization methods, cost reduction, chemical alterations, phytotoxicity
Plant Science
Horticulture
Sterilization (microbiology)
lcsh:Plant culture
biology.organism_classification
01 natural sciences
03 medical and health sciences
chemistry.chemical_compound
030104 developmental biology
Micropropagation
chemistry
Peracetic acid
Gerbera jamesonii
Shoot
Phytotoxicity
lcsh:SB1-1110
010606 plant biology & botany
Subjects
Details
- Language :
- English
- Volume :
- 24
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Ornamental Horticulture
- Accession number :
- edsair.doi.dedup.....02d2ffc5947d3639db8b324649a7ebd5