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Surface functionalisation with viscosity-sensitive BODIPY molecular rotor
- Source :
- Methods and Applications in Fluorescence. 6:034001
- Publication Year :
- 2018
- Publisher :
- IOP Publishing, 2018.
-
Abstract
- Surface functionalisation with viscosity sensitive dyes termed 'molecular rotors' can potentially open up new opportunities in sensing, for example for non-invasive biological viscosity imaging, in studying the effect of shear stress on lipid membranes and in cells, and in imaging contacts between surfaces upon applied pressure. We have functionalised microscope slides with BODIPY-based molecular rotor capable of viscosity sensing via its fluorescence lifetime. We have optimised functionalisation conditions and prepared the slides with the BODIPY rotor attached directly to the surface of glass slides and through polymer linkers of 5 kDa and 40 kDa in mass. The slides were characterised for their sensitivity to viscosity, and used to measure viscosity of supported lipid bilayers during photooxidation, and of giant unilamellar vesicles lying on the surface of the slide. We conclude that our functionalised slides show promise for a variety of viscosity sensing applications.
- Subjects :
- Fluorescence-lifetime imaging microscopy
Materials science
PROTEIN
02 engineering and technology
MEMBRANES
010402 general chemistry
fluorescence microscopy
01 natural sciences
molecular rotors
PHASES
Viscosity
chemistry.chemical_compound
General Materials Science
LIVING CELLS
PROBE
Lipid bilayer
Instrumentation
Spectroscopy
fluorescence lifetime imaging microscopy
chemistry.chemical_classification
Science & Technology
Chemistry, Physical
Vesicle
Chemistry, Analytical
MICROSCOPY
SENSOR
Polymer
021001 nanoscience & nanotechnology
LIVE CELLS
Fluorescence
Atomic and Molecular Physics, and Optics
0104 chemical sciences
Chemistry
Membrane
Chemical engineering
chemistry
Physical Sciences
fluorescent sensors
BODIPY
0210 nano-technology
BEHAVIOR
Subjects
Details
- ISSN :
- 20506120
- Volume :
- 6
- Database :
- OpenAIRE
- Journal :
- Methods and Applications in Fluorescence
- Accession number :
- edsair.doi.dedup.....029f2ec562280e55f09482d4ff0add88