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Complete DNA sequence of the mitochondrial genome of the sea-slug, Aplysia californica: Conservation of the gene order in Euthyneura
- Source :
- Molecular Phylogenetics and Evolution. 38:459-469
- Publication Year :
- 2006
- Publisher :
- Elsevier BV, 2006.
-
Abstract
- We have sequenced and characterized the complete mitochondrial genome of the sea slug, Aplysia californica, an important model organism in experimental biology and a representative of Anaspidea (Opisthobranchia, Gastropoda). The mitochondrial genome of Aplysia is in the small end of the observed sizes of animal mitochondrial genomes (14,117 bp, NCBI Accession No. NC_005827). The Aplysia genome, like most other mitochondrial genomes, encodes genes for 2 ribosomal subunit RNAs (small and large rRNAs), 22 tRNAs, and 13 protein subunits (cytochrome c oxidase subunits 1-3, cytochrome b apoenzyme, ATP synthase subunits 6 and 8, and NADH dehydrogenase subunits 1-6 and 4L). The gene order is virtually identical between opisthobranchs and pulmonates, with the majority of differences arising from tRNA translocations. In contrast, the gene order from representatives of basal gastropods and other molluscan classes is significantly different from opisthobranchs and pulmonates. The Aplysia genome was compared to all other published molluscan mitochondrial genomes and phylogenetic analyses were carried out using a concatenated protein alignment. Phylogenetic analyses using maximum likelihood based analyses of the well aligned regions of the protein sequences support both monophyly of Euthyneura (a group including both the pulmonates and opisthobranchs) and Opisthobranchia (as a more derived group). The Aplysia mitochondrial genome sequenced here will serve as an important platform in both comparative and neurobiological studies using this model organism.
- Subjects :
- Mitochondrial DNA
animal structures
Molecular Sequence Data
Biology
DNA, Mitochondrial
Genome
RNA, Transfer
Phylogenetics
Aplysia
Gene Order
Genetics
Animals
NADH, NADPH Oxidoreductases
Molecular Biology
Gene
Phylogeny
Ecology, Evolution, Behavior and Systematics
Gene Rearrangement
Base Sequence
Cytochrome b
Euthyneura
Genes, rRNA
Mitochondrial Proton-Translocating ATPases
Ribosomal RNA
biology.organism_classification
Genes, Mitochondrial
Transfer RNA
Nucleic Acid Conformation
Subjects
Details
- ISSN :
- 10557903
- Volume :
- 38
- Database :
- OpenAIRE
- Journal :
- Molecular Phylogenetics and Evolution
- Accession number :
- edsair.doi.dedup.....029d9a84e50dba1590faf88e8f0da6a1