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The sortilin cytoplasmic tail conveys Golgi-endosome transport and binds the VHS domain of the GGA2 sorting protein
- Source :
- Nielsen, M S, Madsen, P S, Christensen, E I, Nykjær, A, Gliemann, J, Kasper, D, Pohlmann, R & Pedersen, J C M 2001, ' The sortilin cytoplasmic tail conveys Golgi-endosome transport and binds the VHS domain of the GGA2 sorting protein ', E M B O Journal, vol. 20, pp. 2180-2190 ., Nielsen, M S, Madsen, P, Christensen, E I, Nykjær, A, Gliemann, J, Kasper, D, Pohlmann, R & nullPetersenC., M 2001, ' The sortilin cytoplasmic tail conveys Golgi-endosome transport and binds the VHS domain of the GGA2 sorting protein ' E M B O Journal, vol. 20, pp. 2180-2190 ., Aarhus University
- Publication Year :
- 2001
-
Abstract
- Sortilin belongs to a growing family of multiligand type-1 receptors with homology to the yeast receptor Vps10p. Based on structural features and sortilin's intracellular predominance, we have proposed it to be a sorting receptor for ligands in the synthetic pathway as well as on the cell membrane. To test this hypothesis we examine here the cellular trafficking of chimeric receptors containing constructs of the sortilin tail. We report that sorting signals conforming to YXX and dileucine motifs mediate rapid endocytosis of sortilin chimeras, which subsequently travel to the trans-Golgi network, showing little or no recycling. Furthermore, we found that cation-independent mannose 6-phosphate receptor (MPR300)-sortilin chimeras, expressed in mannose 6-phosphate receptor knockout cells, were almost as efficient as MPR300 itself for transport of newly synthesized beta-hexosaminidase and beta-glucuronidase to lysosomes, and established that the sortilin tail contains potent signals for Golgi-endosome sorting. Finally, we provide evidence suggesting that sortilin is the first example of a mammalian receptor targeted by the recently described GGA family of cytosolic sorting proteins, which condition the Vps10p-mediated sorting of yeast carboxypeptidase Y.
- Subjects :
- Cytoplasm
Saccharomyces cerevisiae Proteins
Endosome
Recombinant Fusion Proteins
Amino Acid Motifs
Vesicular Transport Proteins
Gene Expression
Golgi Apparatus
Nerve Tissue Proteins
Receptors, Cell Surface
CHO Cells
Endosomes
Biology
Transfection
Endocytosis
Article
General Biochemistry, Genetics and Molecular Biology
Fungal Proteins
Cell membrane
symbols.namesake
Cricetinae
Two-Hybrid System Techniques
GGA1
medicine
Animals
Humans
Amino Acid Sequence
Receptor
Molecular Biology
Fungal protein
Membrane Glycoproteins
General Immunology and Microbiology
General Neuroscience
Proteins
Receptors, Interleukin-2
Golgi apparatus
Protein Structure, Tertiary
Transport protein
Cell biology
Adaptor Proteins, Vesicular Transport
Protein Transport
medicine.anatomical_structure
Biochemistry
symbols
Carrier Proteins
Protein Binding
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Nielsen, M S, Madsen, P S, Christensen, E I, Nykjær, A, Gliemann, J, Kasper, D, Pohlmann, R & Pedersen, J C M 2001, ' The sortilin cytoplasmic tail conveys Golgi-endosome transport and binds the VHS domain of the GGA2 sorting protein ', E M B O Journal, vol. 20, pp. 2180-2190 ., Nielsen, M S, Madsen, P, Christensen, E I, Nykjær, A, Gliemann, J, Kasper, D, Pohlmann, R & nullPetersenC., M 2001, ' The sortilin cytoplasmic tail conveys Golgi-endosome transport and binds the VHS domain of the GGA2 sorting protein ' E M B O Journal, vol. 20, pp. 2180-2190 ., Aarhus University
- Accession number :
- edsair.doi.dedup.....029a192919559302a41885ae28cceece