Back to Search
Start Over
Lack of effects of recombinant human GH on spermatogenesis in the adult cynomolgus monkey (Macaca fascicularis)
- Source :
- European journal of endocrinology. 140(4)
- Publication Year :
- 1999
-
Abstract
- OBJECTIVE: The effects on male reproductive parameters after 1 year of treatment with recombinant human GH to the cynomolgus monkey were investigated. DESIGN: Twenty-four male cynomolgus monkeys were given daily subcutaneous doses of 0 (vehicle) (n=7), 0.4 (n=5), 2.0 (n=5) and 10.0 (n=7) IU/kg bodyweight for 52 weeks. At completion of the treatment period two control and two high-dose animals were left for a 12-week treatment-free period. METHODS: Before and during the treatment period and during the recovery period, sperm analyses, testicular volume measurements and hormone analyses of prolactin (PRL), LH, FSH, testosterone and IGF-I in serum, and analysis of serum antibodies against human GH were performed. Testicular morphology was monitored by biopsies, predose and on day 15 of the study, and with light microscopy on organ samples collected at time of death, at the end of the treatment, and during recovery periods respectively. RESULTS: Of all studied parameters, alterations were observed only in serum levels of IGF-I and PRL. IGF-I showed a dose-dependent increase throughout the treatment, with a normalisation during the treatment-free period. PRL decreased significantly in animals given 10.0IU/kg per day from week 14 of treatment and throughout the study but with a normalisation upon cessation of treatment. Spermatogenesis, as judged from semen analysis, testicular volume measurements and testicular morphology was not affected. CONCLUSION: This controlled preclinical study demonstrates that high doses of human GH do not alter male reproductive parameters in a non-human primate model.
- Subjects :
- Male
endocrine system
medicine.medical_specialty
Time Factors
Endocrinology, Diabetes and Metabolism
Period (gene)
Semen analysis
Endocrinology
biology.animal
Internal medicine
Testis
medicine
Animals
Primate
Spermatogenesis
Testosterone
medicine.diagnostic_test
biology
Sperm Count
Human Growth Hormone
Body Weight
General Medicine
Organ Size
Luteinizing Hormone
Sperm
Prolactin
Macaca fascicularis
Growth Hormone
Hormone
Subjects
Details
- ISSN :
- 08044643
- Volume :
- 140
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- European journal of endocrinology
- Accession number :
- edsair.doi.dedup.....02915f4e6a05c74f3284e9bf15768f14