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Complete Mitochondrial Genome of a Gymnosperm, Sitka Spruce (Picea sitchensis), Indicates a Complex Physical Structure
- Source :
- Genome Biology and Evolution
- Publication Year :
- 2020
- Publisher :
- Oxford University Press (OUP), 2020.
-
Abstract
- Plant mitochondrial genomes vary widely in size. Although many plant mitochondrial genomes have been sequenced and assembled, the vast majority are of angiosperms, and few are of gymnosperms. Most plant mitochondrial genomes are smaller than a megabase, with a few notable exceptions. We have sequenced and assembled the complete 5.5-Mb mitochondrial genome of Sitka spruce (Picea sitchensis), to date, one of the largest mitochondrial genomes of a gymnosperm. We sequenced the whole genome using Oxford Nanopore MinION, and then identified contigs of mitochondrial origin assembled from these long reads based on sequence homology to the white spruce mitochondrial genome. The assembly graph shows a multipartite genome structure, composed of one smaller 168-kb circular segment of DNA, and a larger 5.4-Mb single component with a branching structure. The assembly graph gives insight into a putative complex physical genome structure, and its branching points may represent active sites of recombination.
- Subjects :
- AcademicSubjects/SCI01140
0106 biological sciences
Mitochondrial DNA
gymnosperms
organelle
Sitka spruce
Sequence assembly
Biology
01 natural sciences
Genome
ABySS
03 medical and health sciences
Gymnosperm
Genetics
Picea
Ecology, Evolution, Behavior and Systematics
030304 developmental biology
0303 health sciences
Molecular Structure
Contig
fungi
AcademicSubjects/SCI01130
sequencing
biology.organism_classification
Genome Report
Multipartite
Evolutionary biology
Minion
Genome, Mitochondrial
genome assembly
Nanopore sequencing
Genome, Plant
010606 plant biology & botany
Subjects
Details
- ISSN :
- 17596653
- Volume :
- 12
- Database :
- OpenAIRE
- Journal :
- Genome Biology and Evolution
- Accession number :
- edsair.doi.dedup.....027a82f1dfdce89764fc21a7eba4c7bc