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Wogonin prevents lipopolysaccharide-induced acute lung injury and inflammation in mice via peroxisome proliferator-activated receptor gamma-mediated attenuation of the nuclear factor-kappaB pathway
- Source :
- Immunology. 143:241-257
- Publication Year :
- 2014
- Publisher :
- Wiley, 2014.
-
Abstract
- Acute lung injury (ALI) from a variety of clinical disorders, characterized by diffuse inflammation, is a cause of acute respiratory failure that develops in patients of all ages. Previous studies reported that wogonin, a flavonoid-like chemical compound which was found in Scutellaria baicalensis, has anti-inflammatory effects in several inflammation models, but not in ALI. Here, the in vivo protective effect of wogonin in the amelioration of lipopolysaccharide (LPS) -induced lung injury and inflammation was assessed. In addition, the in vitro effects and mechanisms of wogonin were studied in the mouse macrophage cell lines Ana-1 and RAW264.7. In vivo results indicated that wogonin attenuated LPS-induced histological alterations. Peripheral blood leucocytes decreased in the LPS-induced group, which was ameliorated by wogonin. In addition, wogonin inhibited the production of several inflammatory cytokines, including tumour necrosis factor-α, interleukin-1β (IL-1β) and IL-6, in the bronchoalveolar lavage fluid and lung tissues after LPS challenge, while the peroxisome proliferator-activated receptor γ (PPARγ) inhibitor GW9662 reversed these effects. In vitro results indicated that wogonin significantly decreased the secretion of IL-6, IL-1β and tumour necrosis factor-α in Ana-1 and RAW264.7 cells, which was suppressed by transfection of PPARγ small interfering RNA and GW9662 treatment. Moreover, wogonin activated PPARγ, induced PPARγ-mediated attenuation of the nuclear translocation and the DNA-binding activity of nuclear factor-κB in vivo and in vitro. In conclusion, all of these results showed that wogonin may serve as a promising agent for the attenuation of ALI-associated inflammation and pathology by regulating the PPARγ-involved nuclear factor-κB pathway.
- Subjects :
- Lipopolysaccharides
Time Factors
Acute Lung Injury
Immunology
Anti-Inflammatory Agents
Peroxisome proliferator-activated receptor
Inflammation
Lung injury
Pharmacology
Transfection
Cell Line
Proinflammatory cytokine
Mice
chemistry.chemical_compound
Wogonin
In vivo
medicine
Animals
Immunology and Allergy
Lung
chemistry.chemical_classification
biology
business.industry
Macrophages
NF-kappa B
Original Articles
Pneumonia
biology.organism_classification
NFKB1
Mice, Inbred C57BL
PPAR gamma
Disease Models, Animal
Gene Expression Regulation
chemistry
Flavanones
Cytokines
Scutellaria baicalensis
Female
RNA Interference
Inflammation Mediators
medicine.symptom
business
Signal Transduction
Subjects
Details
- ISSN :
- 00192805
- Volume :
- 143
- Database :
- OpenAIRE
- Journal :
- Immunology
- Accession number :
- edsair.doi.dedup.....027962f0cd5cd0b9adcef02bb81f6380