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On the control of dispersion interactions between biological membranes and protein coated biointerfaces
- Source :
- Journal of Colloid and Interface Science, Journal of Colloid and Interface Science, Elsevier, 2021, 598, pp.464-473. ⟨10.1016/j.jcis.2021.02.078⟩, Journal of Colloid and Interface Science, 2021, 598, pp.464-473. ⟨10.1016/j.jcis.2021.02.078⟩
- Publication Year :
- 2021
- Publisher :
- Elsevier BV, 2021.
-
Abstract
- Hypothesis Interaction of cellular membranes with biointerfaces is of vital importance for a number of medical devices and implants. Adhesiveness of these surfaces and cells is often regulated by depositing a layer of bovine serum albumin (BSA) or other protein coatings. However, anomalously large separations between phospholipid membranes and the biointerfaces in various conditions and buffers have been observed, which could not be understood using available theoretical arguments. Methods Using the Lifshitz theory, we here evaluate the distance-dependent Hamaker coefficient describing the dispersion interaction between a biointerface and a membrane to understand the relative positioning of two surfaces. Our theoretical modeling is supported by experiments where the biointerface is represented by a glass substrate with deposited BSA and protein layers. These biointerfaces are allowed to interact with giant unilamellar vesicles decorated with polyethylene glycol (PEG) using PEG lipids to mimic cellular membranes and their pericellular coat. Results We demonstrate that careful treatment of the van der Waals interactions is critical for explaining the lack of adhesiveness of the membranes with protein-decorated biointerfaces. We show that BSA alone indeed passivates the glass, but depositing an additional protein layer on the surface BSA, or producing multiple layers of proteins and BSA results in repulsive dispersion forces responsible for 100 nm large equilibrium separations between the two surfaces.
- Subjects :
- Biointerface
02 engineering and technology
Polyethylene glycol
010402 general chemistry
01 natural sciences
London dispersion force
Polyethylene Glycols
Biomaterials
chemistry.chemical_compound
symbols.namesake
Colloid and Surface Chemistry
[PHYS.COND]Physics [physics]/Condensed Matter [cond-mat]
Bovine serum albumin
ComputingMilieux_MISCELLANEOUS
biology
Chemistry
Physics
Vesicle
Adhesiveness
Serum Albumin, Bovine
Biological membrane
021001 nanoscience & nanotechnology
0104 chemical sciences
Surfaces, Coatings and Films
Electronic, Optical and Magnetic Materials
Membrane
biology.protein
symbols
Biophysics
Glass
van der Waals force
Biointerfaces
Dispersion forces
Lifshitz theory
Van der Waals interactions
Reflection interference contrast microscopy (RICM)
0210 nano-technology
Subjects
Details
- ISSN :
- 00219797 and 10957103
- Volume :
- 598
- Database :
- OpenAIRE
- Journal :
- Journal of Colloid and Interface Science
- Accession number :
- edsair.doi.dedup.....027604fdce48c9af3367919aa0cc0a9e