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Cellulolytic bacteria isolation, screening and optimization of enzyme production from vermicompost of paper cup waste
- Source :
- Waste Management. 116:58-65
- Publication Year :
- 2020
- Publisher :
- Elsevier BV, 2020.
-
Abstract
- In the current scenario, used paper cups are disposed of without any proper treatment, thereby damaging the environment. Hence, the vermicomposting technique is preferred for managing these wastes. The ability of bacterial strains on cellulase enzyme (Endoglucanase, exoglucanase and β-glucosidase) production at altered pH and temperatures were focused in this study. Among nine bacterial strains Acinetobacter baumannii was found to have high enzyme activity. HPLC analysis confirms that about 45% of cellulose degradation occurred due to the action of bacterial consortia at 37 °C with pH 7. The overall period required for degradation takes only three months with the help of bacterial consortia while comparing to our previous study, which takes six months. The insilico study on three cellulose-degrading enzymes sequence were retrieved from NCBI, and analysed for multiple sequence alignment and phylogenetic tree construction. From the analysis, the endoglucanase SVK46152 (Acinetobacter baumannii) sequence got docked with cellopentaose with a high score value −11.07. Thereby we confirm that organism Acinetobacter baumannii was effective in paper cup degradation.
- Subjects :
- 020209 energy
02 engineering and technology
Cellulase
010501 environmental sciences
engineering.material
01 natural sciences
0202 electrical engineering, electronic engineering, information engineering
Food science
Cellulose
Waste Management and Disposal
Phylogeny
0105 earth and related environmental sciences
chemistry.chemical_classification
Multiple sequence alignment
Bacteria
biology
Phylogenetic tree
beta-Glucosidase
biology.organism_classification
Enzyme assay
Acinetobacter baumannii
Enzyme
chemistry
biology.protein
engineering
Vermicompost
Subjects
Details
- ISSN :
- 0956053X
- Volume :
- 116
- Database :
- OpenAIRE
- Journal :
- Waste Management
- Accession number :
- edsair.doi.dedup.....0275e2ea081663891155a43660871cb4