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Rapid and simple determination of fleroxacin in rat plasma using a solid-phase extraction column
- Source :
- Journal of Chromatography B: Biomedical Sciences and Applications. 703:279-283
- Publication Year :
- 1997
- Publisher :
- Elsevier BV, 1997.
-
Abstract
- We studied the use of high-performance liquid chromatography (HPLC) with a spectroflurometric detector, using a solid-phase extraction column (Bond Elut cartridge column), for the simple, rapid and sensitive determination of plasma fleroxacin (FLRX) levels in rats. Extracted aliquots were analyzed by HPLC, using a reverse phase octadecyl silica column. The analytical mean recovery of FLRX added to the blank plasma averaged 101.4%. The detection limit was 58 ng/ml in the plasma. The reproducibilities (C.V.) were 0.50-3.22% in the within-day assay and 2.87 C.V.% in the between-day assay, indicating that the analysis method was effective in the determination of FLRX plasma levels.
- Subjects :
- Male
Time Factors
Fleroxacin
Administration, Oral
Sensitivity and Specificity
High-performance liquid chromatography
Rats, Sprague-Dawley
Cartridge
Anti-Infective Agents
medicine
Animals
Solid phase extraction
Chromatography, High Pressure Liquid
Antibacterial agent
Detection limit
Chromatography
Chemistry
Osmolar Concentration
Extraction (chemistry)
Reproducibility of Results
General Chemistry
Silicon Dioxide
Circadian Rhythm
Rats
Quantitative analysis (chemistry)
medicine.drug
Subjects
Details
- ISSN :
- 03784347
- Volume :
- 703
- Database :
- OpenAIRE
- Journal :
- Journal of Chromatography B: Biomedical Sciences and Applications
- Accession number :
- edsair.doi.dedup.....025818eae95bedd5b3547196aca0060c