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Feline calicivirus- and murine norovirus-induced COX-2/PGE2 signaling pathway has proviral effects
- Source :
- PLoS ONE, PLoS ONE, Vol 13, Iss 7, p e0200726 (2018)
- Publication Year :
- 2018
-
Abstract
- Cyclooxygenases (COXs)/prostaglandin E2 (PGE2) signaling pathways are known to modulate a variety of homeostatic processes and are involved in various pathophysiological conditions. COXs/PGE2 signaling pathways have also been demonstrated to have proviral or antiviral effects, which appeared different even in the same virus family. A porcine sapovirus Cowden strain, a member of genus Sapovirus within the Caliciviridae family, induces strong COX-2/PGE2 but transient COX-1/PGE2 signaling to enhance virus replication. However, whether infections of other viruses in the different genera activate COXs/PGE2 signaling, and thus affect the replication of viruses, remains unknown. In the present study, infections of cells with the feline calicivirus (FCV) F9 strain in the genus Vesivirus and murine norovirus (MNV) CW-1 strain in the genus Norovirus only activated the COX-2/PGE2 signaling in a time-dependent manner. Treatment with pharmacological inhibitors or transfection of small interfering RNAs (siRNAs) against COX-2 enzyme significantly reduced the production of PGE2 as well as FCV and MNV replications. The inhibitory effects of these pharmacological inhibitors against COX-2 enzyme on the replication of both viruses were restored by the addition of PGE2. Silencing of COX-1 via siRNAs and inhibition of COX-1 via an inhibitor also decrease the production of PGE2 and replication of both viruses, which can be attributed to the inhibition COX-1/PGE2 signaling pathway. These data indicate that the COX-2/PGE2 signaling pathway has proviral effects for the replication of FCV and MNV, and pharmacological inhibitors against these enzymes serve as potential therapeutic candidates for treating FCV and MNV infections.
- Subjects :
- 0301 basic medicine
RNA viruses
Small interfering RNA
Viral Diseases
Pulmonology
ved/biology.organism_classification_rank.species
lcsh:Medicine
Virus Replication
Pathology and Laboratory Medicine
Biochemistry
Mice
Proviruses
Medicine and Health Sciences
Small interfering RNAs
lcsh:Science
Feline calicivirus
Analgesics
Viral Genomics
Multidisciplinary
Mammalian Genomics
Drugs
Genomics
Nucleic acids
Infectious Diseases
Medical Microbiology
Viral Pathogens
Viruses
lipids (amino acids, peptides, and proteins)
Signal transduction
Pathogens
Signal Transduction
Research Article
030106 microbiology
Microbial Genomics
Biology
Microbiology
Virus
Dinoprostone
Sapovirus
COX-2 inhibitors
Caliciviruses
03 medical and health sciences
Virology
Genetics
Gene silencing
Animals
Non-coding RNA
Microbial Pathogens
Pharmacology
Biology and life sciences
ved/biology
lcsh:R
Norovirus
Organisms
Membrane Proteins
Calicivirus Infection
biology.organism_classification
Caliciviridae
Viral Replication
Pain management
Gene regulation
030104 developmental biology
RAW 264.7 Cells
Viral replication
Cyclooxygenase 2
Animal Genomics
Respiratory Infections
Cats
Cyclooxygenase 1
RNA
lcsh:Q
Gene expression
Murine norovirus
Calicivirus, Feline
Subjects
Details
- ISSN :
- 19326203
- Volume :
- 13
- Issue :
- 7
- Database :
- OpenAIRE
- Journal :
- PloS one
- Accession number :
- edsair.doi.dedup.....0250884fd15f6973a18105feb58d3adc