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Distinct expression of interleukin (IL)-36α, β and γ, their antagonist IL-36Ra and IL-38 in psoriasis, rheumatoid arthritis and Crohn's disease

Authors :
Franck Morel
Jérôme Amiaud
A. Bourreille
Marie-Astrid Boutet
Bénédicte Brulin
Céline Charrier
Maël Penhoat
M. Rolli‐Derkinderen
Frédéric Blanchard
B. Le Goff
Gaby Palmer
J.-C. Lecron
Géraldine Bart
Solenne Vigne
Cem Gabay
maurice, sandrine
Physiopathologie des Adaptations Nutritionnelles (PhAN)
Institut National de la Recherche Agronomique (INRA)-Université de Nantes (UN)
Service de rhumatologie [Nantes]
Université de Nantes (UN)-Hôtel-Dieu-Centre hospitalier universitaire de Nantes (CHU Nantes)
Laboratoire Inflammation, Tissus épithéliaux et Cytokines (LITEC)
Université de Poitiers
Service Immunologie/Inflammation [CHU Poitiers]
Centre hospitalier universitaire de Poitiers (CHU Poitiers)
Neuropathies du système nerveux entérique et pathologies digestives
implication des cellules gliales entériques
Université de Nantes (UN)-Institut National de la Santé et de la Recherche Médicale (INSERM)
Service d'hépato-gastroentérologie [CHU Nantes]
Centre hospitalier universitaire de Nantes (CHU Nantes)
Division of Rheumatology [Geneva, Switzerland]
Geneva University Hospital, Geneva-Department of Internal Medicine Specialties [Geneva, Switzerland]
This work was supported by Inserm, the Arthritis Foundation and by a grant from the Swiss National Science Foundation (310030_135195 to CG). M.A.B. was a recipient of a fellowship from the French Ministry of Research.
Université de Nantes - UFR de Médecine et des Techniques Médicales (UFR MEDECINE)
Université de Nantes (UN)-Université de Nantes (UN)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)
Université de Genève (UNIGE)
ROLLI-DERKINDEREN, MALVYNE
Université de Genève = University of Geneva (UNIGE)
Source :
Clinical and Experimental Immunology, Vol. 184, No 2 (2016) pp. 159-173, Clinical and Experimental Immunology, Clinical and Experimental Immunology, Wiley, 2016, 184 (2), pp.159-173. ⟨10.1111/cei.12761⟩, Clinical and experimental immunology
Publication Year :
2016

Abstract

Summary Interleukin (IL)-36α, IL-36β and IL-36γ are expressed highly in skin and are involved in the pathogenesis of psoriasis, while the antagonists IL-36Ra or IL-38, another potential IL-36 inhibitor, limit uncontrolled inflammation. The expression and role of IL-36 cytokines in rheumatoid arthritis (RA) and Crohn's disease (CD) is currently debated. Here, we observed that during imiquimod-induced mouse skin inflammation and in human psoriasis, expression of IL-36α, γ and IL-36Ra, but not IL-36β and IL-38 mRNA, was induced and correlated with IL-1β and T helper type 17 (Th17) cytokines (IL-17A, IL-22, IL-23, CCL20). In mice with collagen-induced arthritis and in the synovium of patients with RA, IL-36α, β, γ, IL-36Ra and IL-38 were all elevated and correlated with IL-1β, CCL3, CCL4 and macrophage colony-stimulating factor (M-CSF), but not with Th17 cytokines. In the colon of mice with dextran sulphate sodium-induced colitis and in patients with CD, only IL-36α, γ and IL-38 were induced at relatively low levels and correlated with IL-1β and IL-17A. We suggest that only a minor subgroup of patients with RA (17–29%) or CD (25%) had an elevated IL-36 agonists/antagonists ratio, versus 93% of patients with psoriasis. By immunohistochemistry, IL-36 cytokines were produced by various cell types in skin, synovium and colonic mucosa such as keratinocytes, CD68+ macrophages, dendritic/Langerhans cells and CD79α+ plasma cells. In primary cultures of monocytes or inflammatory macrophages (M1), IL-36β and IL-36Ra were produced constitutively, but IL-36α, γ and IL-38 were produced after lipopolysaccharide stimulation. These distinct expression profiles may help to explain why only subgroups of RA and CD patients have a potentially elevated IL-36 agonists/antagonists ratio.

Subjects

Subjects :
0301 basic medicine
Keratinocytes
Male
rheumatoid arthritis
Arthritis
[SDV.IMM.II]Life Sciences [q-bio]/Immunology/Innate immunity
Arthritis, Rheumatoid
Mice
Crohn Disease
Immunology and Allergy
Macrophage
Medicine
Intestinal Mucosa
Skin
ddc:616
[SDV.MHEP.RSOA] Life Sciences [q-bio]/Human health and pathology/Rhumatology and musculoskeletal system
Imiquimod
[SDV.MHEP] Life Sciences [q-bio]/Human health and pathology
Synovial Membrane
Interleukin-36
Interleukin
psoriasis
3. Good health
Crohn's disease
[SDV.IMM.IA]Life Sciences [q-bio]/Immunology/Adaptive immunology
[SDV.MHEP.RSOA]Life Sciences [q-bio]/Human health and pathology/Rhumatology and musculoskeletal system
Mice, Inbred DBA
[SDV.IMM.IA] Life Sciences [q-bio]/Immunology/Adaptive immunology
Rheumatoid arthritis
Aminoquinolines
medicine.symptom
Immunology
Plasma Cells
Inflammation
Enzyme-Linked Immunosorbent Assay
Cell Line
03 medical and health sciences
Psoriasis
Animals
Humans
RNA, Messenger
[SDV.IMM.II] Life Sciences [q-bio]/Immunology/Innate immunity
business.industry
Interleukins
Macrophages
[SDV.MHEP.HEG]Life Sciences [q-bio]/Human health and pathology/Hépatology and Gastroenterology
Dendritic Cells
Original Articles
[SDV.MHEP.DERM] Life Sciences [q-bio]/Human health and pathology/Dermatology
medicine.disease
Arthritis, Experimental
[SDV.MHEP.HEG] Life Sciences [q-bio]/Human health and pathology/Hépatology and Gastroenterology
CCL20
Mice, Inbred C57BL
Disease Models, Animal
030104 developmental biology
IL-36
Th17 Cells
Caco-2 Cells
business
[SDV.MHEP.DERM]Life Sciences [q-bio]/Human health and pathology/Dermatology
[SDV.MHEP]Life Sciences [q-bio]/Human health and pathology
Interleukin-1

Details

Language :
English
ISSN :
00099104 and 13652249
Database :
OpenAIRE
Journal :
Clinical and Experimental Immunology, Vol. 184, No 2 (2016) pp. 159-173, Clinical and Experimental Immunology, Clinical and Experimental Immunology, Wiley, 2016, 184 (2), pp.159-173. ⟨10.1111/cei.12761⟩, Clinical and experimental immunology
Accession number :
edsair.doi.dedup.....0226c6cc8a8ee5d44ddbc357174a6bf9
Full Text :
https://doi.org/10.1111/cei.12761⟩