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Endocannabinoid, anandamide in gingival tissue regulates the periodontal inflammation through NF-kappaB pathway inhibition

Authors :
Yumiko Nakajima
Yuichi Izumi
Tomonori Uchimura
Ikuro Maruyama
Kamal Krishna Biswas
Kazuyo Yamaji
Teruto Hashiguchi
Yasushi Furuichi
Ko-ichi Kawahara
Source :
FEBS letters. 580(2)
Publication Year :
2005

Abstract

Anandamide (AEA) exhibits anti-inflammatory effects. However, its role in the periodontal field remains unknown. Here, we found that gingival crevicular fluid contained a detectable level of AEA. The cannabinoid receptors CB1 and CB2 were expressed by human gingival fibroblasts (HGFs), and markedly upregulated under pathological conditions. AEA significantly reduced the production of pro-inflammatory mediators (IL-6, IL-8 and MCP-1) induced by Porphyromonas gingivalis LPS in HGFs, and this effect was attenuated by AM251 and SR144528, selective antagonists of CB1 and CB2, respectively. Moreover, AEA completely blocked LPS-triggered NF-κB activation, implying that AEA may regulate hyperinflammatory reactions in periodontitis.

Details

ISSN :
00145793
Volume :
580
Issue :
2
Database :
OpenAIRE
Journal :
FEBS letters
Accession number :
edsair.doi.dedup.....02226b9bd67b8461c5c70b0f44c50285