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Endocannabinoid, anandamide in gingival tissue regulates the periodontal inflammation through NF-kappaB pathway inhibition
- Source :
- FEBS letters. 580(2)
- Publication Year :
- 2005
-
Abstract
- Anandamide (AEA) exhibits anti-inflammatory effects. However, its role in the periodontal field remains unknown. Here, we found that gingival crevicular fluid contained a detectable level of AEA. The cannabinoid receptors CB1 and CB2 were expressed by human gingival fibroblasts (HGFs), and markedly upregulated under pathological conditions. AEA significantly reduced the production of pro-inflammatory mediators (IL-6, IL-8 and MCP-1) induced by Porphyromonas gingivalis LPS in HGFs, and this effect was attenuated by AM251 and SR144528, selective antagonists of CB1 and CB2, respectively. Moreover, AEA completely blocked LPS-triggered NF-κB activation, implying that AEA may regulate hyperinflammatory reactions in periodontitis.
- Subjects :
- AM251
Lipopolysaccharides
Cannabinoid receptor
Polyunsaturated Alkamides
medicine.medical_treatment
Biophysics
Gingiva
Arachidonic Acids
Pharmacology
Biochemistry
NF-κB
Receptor, Cannabinoid, CB2
chemistry.chemical_compound
Receptor, Cannabinoid, CB1
Structural Biology
Cannabinoid Receptor Modulators
Genetics
medicine
Humans
Receptor
Periodontitis
Molecular Biology
Porphyromonas gingivalis
Cytokine
Cells, Cultured
biology
NF-kappa B
Cell Biology
Anandamide
Gingival Crevicular Fluid
Fibroblasts
biology.organism_classification
Endocannabinoid system
Gingivitis
chemistry
Immunology
lipids (amino acids, peptides, and proteins)
Cannabinoid
medicine.drug
Endocannabinoids
Signal Transduction
Subjects
Details
- ISSN :
- 00145793
- Volume :
- 580
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- FEBS letters
- Accession number :
- edsair.doi.dedup.....02226b9bd67b8461c5c70b0f44c50285