Back to Search
Start Over
Laser-induced collagen remodeling and deposition within the basilar membrane of the mouse cochlea
- Source :
- Journal of Biomedical Optics. 12:021007
- Publication Year :
- 2007
- Publisher :
- SPIE-Intl Soc Optical Eng, 2007.
-
Abstract
- The cochlea is the mammalian organ of hearing. Its predominant vibratory element, the basilar membrane, is tonotopically tuned, based on the spatial variation of its mass and stiffness. The constituent collagen fibers of the basilar membrane affect its stiffness. Laser irradiation can induce collagen remodeling and deposition in various tissues. We tested whether similar effects could be induced within the basilar membrane. Trypan blue was perfused into the scala tympani of anesthetized mice to stain the basilar membrane. We then irradiated the cochleas with a 694-nm pulsed ruby laser at 15 or 180 Jcm(2). The mice were sacrificed 14 to 16 days later and collagen organization was studied. Polarization microscopy revealed that laser irradiation increased the birefringence within the basilar membrane in a dose-dependent manner. Electron microscopy demonstrated an increase in the density of collagen fibers and the deposition of new fibrils between collagen fibers after laser irradiation. As an assessment of hearing, auditory brainstem response (ABR) thresholds were found to increase moderately after 15 Jcm(2) and substantially after 180 Jcm(2). Our results demonstrate that collagen remodeling and new collagen deposition occurs within the basilar membrane after laser irradiation in a similar fashion to that found in other tissues.
- Subjects :
- Biomedical Engineering
Connective tissue
Radiation Dosage
Fibril
Article
law.invention
Biomaterials
Mice
chemistry.chemical_compound
law
Microscopy
otorhinolaryngologic diseases
medicine
Animals
Cochlea
Lasers
Auditory Threshold
Anatomy
Basilar Membrane
Atomic and Molecular Physics, and Optics
Electronic, Optical and Magnetic Materials
Mice, Inbred C57BL
Basilar membrane
medicine.anatomical_structure
chemistry
Transmission electron microscopy
Biophysics
Trypan blue
Collagen
sense organs
Electron microscope
Subjects
Details
- ISSN :
- 10833668
- Volume :
- 12
- Database :
- OpenAIRE
- Journal :
- Journal of Biomedical Optics
- Accession number :
- edsair.doi.dedup.....021d226354ba730273b63e5afbe29075