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The Rhizobial Microbiome from the Tropical Savannah Zones in Northern Côte d’Ivoire

Authors :
Sara Laetitia Elphège Gnangui
Dominique Koua
Romain K Fossou
Claude Ghislaine Kouadjo
Don A. Cowan
Anicet Ebou
Chiguié Estelle Raïssa Amon
Adolphe Zeze
Source :
Microorganisms, Volume 9, Issue 9, Microorganisms, Vol 9, Iss 1842, p 1842 (2021)
Publication Year :
2021
Publisher :
Multidisciplinary Digital Publishing Institute, 2021.

Abstract

Over the past decade, many projects have been initiated worldwide to decipher the composition and function of the soil microbiome, including the African Soil Microbiome (AfSM) project that aims at providing new insights into the presence and distribution of key groups of soil bacteria from across the African continent. In this national study, carried out under the auspices of the AfSM project, we assessed the taxonomy, diversity and distribution of rhizobial genera in soils from the tropical savannah zones in Northern Côte d’Ivoire. Genomic DNA extracted from seven sampled soils was analyzed by sequencing the V4-V5 variable region of the 16S rDNA using Illumina’s MiSeq platform. Subsequent bioinformatic and phylogenetic analyses showed that these soils harbored 12 out of 18 genera of Proteobacteria harboring rhizobia species validly published to date and revealed for the first time that the Bradyrhizobium genus dominates in tropical savannah soils, together with Microvirga and Paraburkholderia. In silico comparisons of different 16S rRNA gene variable regions suggested that the V5-V7 region could be suitable for differentiating rhizobia at the genus level, possibly replacing the use of the V4-V5 region. These data could serve as indicators for future rhizobial microbiome explorations and for land-use decision-making.

Details

Language :
English
ISSN :
20762607
Database :
OpenAIRE
Journal :
Microorganisms
Accession number :
edsair.doi.dedup.....0212888cbd7a16f68262bb7b821d4afa
Full Text :
https://doi.org/10.3390/microorganisms9091842