Characterization of the copper resistance mechanism and bioremediation potential of an Acinetobacter calcoaceticus strain isolated from copper mine sludge

Bioremediation is one of the most effective ways for removal of heavy metals and restoration of contaminated sites. This study investigated the copper (Cu) resistance mechanism and bioremediation potential of an Acinetobacter calcoaceticus strain KW3 isolated from sludge of Cu mine. The effect of Cu concentrations on the bacterial growth, biomass, and adsorption capacity, as well as the effect of contact time on the adsorption process was evaluated in a batch biosorption test. The strain exhibited strong tolerance of Cu, and the minimal inhibitory concentration was around 400 mg Cu2+ L-1, at which the maximum adsorption capacity was 14.1 mg g-1 dry cell mass. Cell walls and intracellular soluble components adsorbed 51.2% and 46.6% of Cu2+, respectively, suggesting that the strain not only adsorbed Cu2+ on the surface but also metastasized ions into cells. The adsorption and kinetic data were well fitted with Freundlich isotherm and Pseudo-second-order models, suggesting that cell surface had a high affinity for Cu2+ and the chemisorption could be the main adsorption mechanism. Scanning electron microscope and Fourier transform infrared spectroscopy analysis revealed that hydroxyl, carboxylic, amide, sulfate, and phosphate on cell walls might be involved in the biosorption process. Two-dimensional gel electrophoresis and MALDI-TOF/TOF mass spectrometry revealed that some oxidoreductases, in particular Cu resistance protein A (CopA) expression levels, were upregulated. Antioxidant defense and P1B-type ATPases CopA efflux might play a crucial role in maintaining cellular homeostasis and intracellular detoxification. To our knowledge, this is the first time that Cu resistance mechanisms, especially intracellular enzymatic mechanisms, were identified in an A. calcoaceticus KW3 strain.