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A high-throughput polymer microarray approach for identifying defined substrates for mesenchymal stem cells
- Source :
- Duffy, C R E, Zhang, R, How, S-E, Lilienkampf, A, Tourniaire, G, Hu, W, West, C C, de Sousa, P & Bradley, M 2014, ' A high-throughput polymer microarray approach for identifying defined substrates for mesenchymal stem cells ', Biomaterials science, vol. 2, no. 11, pp. 1683-1692 . https://doi.org/10.1039/c4bm00112e
- Publication Year :
- 2014
-
Abstract
- Mesenchymal stem cells (MSCs) hold great promise in regenerative medicine due to their wide multilineage potential as well as their ability to suppress/modulate the immune response. Maintaining these cells in vitro and expanding them on a clinically relevant scale remains a challenge that needs to be addressed to realise their full potential. Current culture methods for MSCs typically rely on animal sourced substrates and often result in a heterogeneous population of cells with varying degrees of differentiation capacity. Here, a high-throughput platform was used to identify synthetic substrates for MSC culture that not only facilitated growth but also maintained the MSC phenotype. Two polymers, PU157 (synthesised from poly(butyleneglycol) and 4,4'-methylenediphenyldiisocyanate with 3-(dimethylamino)-1,2-propanediol as a chain extender) and PA338 (N-methylaniline modified poly(methylmethacrylate-co-glycidylmethacrylate)) were able to maintain the growth and phenotype of human embryonic derived mesenchymal progenitors (hES-MPs) and adipose derived MSCs (ADMSCs) for five and ten passages, respectively. Cell phenotype and multipotency were confirmed by flow cytometry analysis of ten MSC markers and differentiation analysis. These new polymer substrates provide a chemically defined synthetic surface for efficient, long-term MSC culture.
- Subjects :
- Stromal cell
BONE-MARROW
Biomedical Engineering
Clinical uses of mesenchymal stem cells
Biology
Regenerative medicine
Flow cytometry
HUMAN ADIPOSE-TISSUE
CULTURE
HUMAN UMBILICAL-CORD
medicine
General Materials Science
Progenitor cell
MATRIX-MEDIATED RETENTION
PRECURSORS
medicine.diagnostic_test
Mesenchymal stem cell
MSCS
Embryonic stem cell
Cell biology
medicine.anatomical_structure
DIFFERENTIATION
STROMAL CELLS
Immunology
EX-VIVO EXPANSION
Bone marrow
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Duffy, C R E, Zhang, R, How, S-E, Lilienkampf, A, Tourniaire, G, Hu, W, West, C C, de Sousa, P & Bradley, M 2014, ' A high-throughput polymer microarray approach for identifying defined substrates for mesenchymal stem cells ', Biomaterials science, vol. 2, no. 11, pp. 1683-1692 . https://doi.org/10.1039/c4bm00112e
- Accession number :
- edsair.doi.dedup.....01e0849c73b9ec5bf72de5ef8643b6dd