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A thermoresponsive and chemically defined hydrogel for long-term culture of human embryonic stem cells
- Source :
- Nature Communications, Zhang, R, Mjoseng, H K, Hoeve, M A, Bauer, N G, Pells, S, Besseling, R, Velugotla, S, Tourniaire, G, Kishen, R E B, Tsenkina, Y, Armit, C, Duffy, C R E, Helfen, M, Edenhofer, F, de Sousa, P A & Bradley, M 2013, ' A thermoresponsive and chemically defined hydrogel for long-term culture of human embryonic stem cells ', Nature Communications, vol. 2013, no. 4, 1335 . https://doi.org/10.1038/ncomms2341, Nature Communications; Vol 4
- Publication Year :
- 2013
- Publisher :
- Springer Science and Business Media LLC, 2013.
-
Abstract
- Cultures of human embryonic stem cell typically rely on protein matrices or feeder cells to support attachment and growth, while mechanical, enzymatic or chemical cell dissociation methods are used for cellular passaging. However, these methods are ill defined, thus introducing variability into the system, and may damage cells. They also exert selective pressures favouring cell aneuploidy and loss of differentiation potential. Here we report the identification of a family of chemically defined thermoresponsive synthetic hydrogels based on 2-(diethylamino)ethyl acrylate, which support long-term human embryonic stem cell growth and pluripotency over a period of 2–6 months. The hydrogels permitted gentle, reagent-free cell passaging by virtue of transient modulation of the ambient temperature from 37 to 15 °C for 30 min. These chemically defined alternatives to currently used, undefined biological substrates represent a flexible and scalable approach for improving the definition, efficacy and safety of human embryonic stem cell culture systems for research, industrial and clinical applications.<br />To transfer cultured human embryonic stem cells (hESCs) between culture dishes, cells need to be released using mechanical, enzymatic or chemical means, which can damage cells. Zhang et al. describe a thermomodulatable hydrogel that allows gentle, reagent-free cell passaging for the long-term culture of hESCs.
- Subjects :
- Time Factors
Cell
Cell Culture Techniques
General Physics and Astronomy
02 engineering and technology
Biology
Biophysical Phenomena
Hydrogel, Polyethylene Glycol Dimethacrylate
Article
General Biochemistry, Genetics and Molecular Biology
Colony-Forming Units Assay
03 medical and health sciences
Cell Adhesion
medicine
Humans
Cells, Cultured
Embryonic Stem Cells
030304 developmental biology
0303 health sciences
Multidisciplinary
Temperature
General Chemistry
021001 nanoscience & nanotechnology
Embryonic stem cell
Culture Media
Cell biology
Drug Combinations
medicine.anatomical_structure
embryonic structures
Electrophoresis, Polyacrylamide Gel
Proteoglycans
Collagen
Laminin
Stress, Mechanical
0210 nano-technology
Subjects
Details
- ISSN :
- 20411723
- Volume :
- 4
- Database :
- OpenAIRE
- Journal :
- Nature Communications
- Accession number :
- edsair.doi.dedup.....01de6377d4f719839b470a3a60017d8a