Back to Search
Start Over
GTPase regulator associated with the focal adhesion kinase (GRAF) transcript was down-regulated in patients with myeloid malignancies
- Source :
- Journal of Experimental & Clinical Cancer Research : CR, Journal of Experimental & Clinical Cancer Research, Vol 29, Iss 1, p 111 (2010)
- Publication Year :
- 2010
- Publisher :
- Springer Science and Business Media LLC, 2010.
-
Abstract
- Background GTPase regulator associated with the focal adhesion kinase (GRAF), a putative tumor suppressor gene, is found inactivated in hematopoietic malignancies by either genetic or epigenetic abnormalities. However, the expression level of GRAF gene has not yet been studied in leukemia. The aim of this study was to investigate the expression level of GRAF gene in those patients with myeloid malignancies including acute myeloid leukemia (AML), myelodysplastic syndrome (MDS) and chronic myeloid leukemia (CML). Methods The expression levels of GRAF transcript were determined in 94 patients using real-time quantitative PCR (RQ-PCR). Clinical and laboratory data of these patients were collected and analyzed. Results The significantly decreased level of GRAF transcript was observed in three myeloid malignancies compared to controls. Within AML, there was no difference in the level of GRAF transcript among different FAB subtypes (P > 0.05). Difference was not observed in the amount of GRAF mRNA between CML at chronic phase and controls. As CML progressed, GRAF transcript significantly decreased. In MDS, three cases with 5q deletion had lower GRAF transcript than four without 5q deletion (median 0.76 vs 2.99) (P > 0.05). Conclusion our results demonstrate that the GRAF transcript is decreased in myeloid malignancies.
- Subjects :
- Adult
Male
Cancer Research
Myeloid
Adolescent
Tumor suppressor gene
Down-Regulation
Biology
lcsh:RC254-282
Immunophenotyping
Young Adult
Leukemia, Myelogenous, Chronic, BCR-ABL Positive
hemic and lymphatic diseases
medicine
Humans
RNA, Messenger
Child
Aged
Aged, 80 and over
Chromosome Aberrations
Reverse Transcriptase Polymerase Chain Reaction
Research
Myelodysplastic syndromes
GTPase-Activating Proteins
Myeloid leukemia
Middle Aged
lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens
medicine.disease
Leukemia, Myeloid, Acute
Haematopoiesis
Leukemia
medicine.anatomical_structure
Real-time polymerase chain reaction
Oncology
Child, Preschool
Myelodysplastic Syndromes
Cytogenetic Analysis
Cancer research
Female
Subjects
Details
- ISSN :
- 17569966
- Volume :
- 29
- Database :
- OpenAIRE
- Journal :
- Journal of Experimental & Clinical Cancer Research
- Accession number :
- edsair.doi.dedup.....01b63cac16647ba9766c963a81937bd4