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Generation of a tenascin-C-CreER2 knockin mouse line for conditional DNA recombination in renal medullary interstitial cells
- Source :
- PLoS ONE, Vol 8, Iss 11, p e79839 (2013), PLoS ONE
- Publication Year :
- 2013
- Publisher :
- Public Library of Science (PLoS), 2013.
-
Abstract
- Renal medullary interstitial cells (RMIC) are specialized fibroblast-like cells that exert important functions in maintaining body fluid homeostasis and systemic blood pressure. Here, we generated a RMIC specific tenascin-C promoter driven inducible CreER2 knockin mouse line with an EGFP reporter. Similar as endogenous tenascin-C expression, the reporter EGFP expression in the tenascin-C-CreER2(+/-) mice was observed in the inner medulla of the kidney, and co-localized with COX2 but not with AQP2 or AQP1, suggesting selective expression in RMICs. After recombination (tenascin-C-CreER2(+/-)/ROSA26-lacZ(+/-) mice + tamoxifen), β-gal activity was restricted to the cells in the inner medulla of the kidney, and didn't co-localize with AQP2, consistent with selective Cre recombinase activity in RMICs. Cre activity was not obvious in other major organs or without tamoxifen treatment. This inducible RMIC specific Cre mouse line should therefore provide a novel tool to manipulate genes of interest in RMICs.
- Subjects :
- Male
Transgene
Green Fluorescent Proteins
Cre recombinase
lcsh:Medicine
Mice, Transgenic
In situ hybridization
Green fluorescent protein
Mice
Genes, Reporter
medicine
Animals
Gene Knock-In Techniques
Transgenes
Promoter Regions, Genetic
lcsh:Science
Crosses, Genetic
Kidney
Kidney Medulla
Multidisciplinary
Aquaporin 2
biology
Aquaporin 1
Integrases
Tenascin C
lcsh:R
Tenascin
Fibroblasts
Molecular biology
Founder Effect
Tamoxifen
medicine.anatomical_structure
Gene Expression Regulation
Lac Operon
Cyclooxygenase 2
Renal physiology
embryonic structures
biology.protein
Female
lcsh:Q
Homeostasis
Research Article
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 8
- Issue :
- 11
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....01b5e81ef1608d0f15c073cba9aad5ca