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Molecular and Immunological Characterization of Gluten Proteins Isolated from Oat Cultivars That Differ in Toxicity for Celiac Disease

Authors :
Ana Real
Francisco L. Merchan
Javier Gil-Humanes
Isabel Comino
M.I. Torres
María J. Giménez
Miguel Ángel López-Casado
Laura de Lorenzo
Fernando Pistón
Carolina Sousa
Francisco Barro
Angel Cebolla
Universidad de Sevilla. Departamento de Microbiología y Parasitología
[Real,A
Comino,I
Lorenzo,L de
Merchán,F
Sousa,C] Departamento de Microbiología y Parasitología, Facultad de Farmacia, Universidad de Sevilla, Sevilla, Spain. [Gil-Humanes,J
Giménez,MJ
Barro,F
Pistón,F] Instituto de Agricultura Sostenible (C.S.I.C.), Córdoba, Spain. [López-Casado,MA] Hospital Virgen de las Nieves, Granada, Spain. [Torres,MI] Departamento de Biología Experimental, Campus Universitario Las Lagunillas, Jaén, Spain. [Cebolla,A] Biomedal S.L., Sevilla, Spain.
This work was supported by the Spanish Ministerio de Economíaa y Competitividad (MINECO,Programa INNPACTO, IPT-2011-1321-010000) and the European Regional Development Fund (FEDER). FP is supported by a Ramón y Cajal research contract from the MINECO (RYC-2010-07345) .
Source :
idUS. Depósito de Investigación de la Universidad de Sevilla, instname, Digital.CSIC. Repositorio Institucional del CSIC, PLoS ONE, Vol 7, Iss 12, p e48365 (2012), PLoS ONE
Publication Year :
2012
Publisher :
Public Library of Science, 2012.

Abstract

Real, Ana et al.<br />A strict gluten-free diet (GFD) is the only currently available therapeutic treatment for patients with celiac disease (CD). Traditionally, treatment with a GFD has excluded wheat, barley and rye, while the presence of oats is a subject of debate. The most-recent research indicates that some cultivars of oats can be a safe part of a GFD. In order to elucidate the toxicity of the prolamins from oat varieties with low, medium, and high CD toxicity, the avenin genes of these varieties were cloned and sequenced, and their expression quantified throughout the grain development. At the protein level, we have accomplished an exhaustive characterization and quantification of avenins by RP-HPLC and an analysis of immunogenicity of peptides present in prolamins of different oat cultivars. Avenin sequences were classified into three different groups, which have homology with S-rich prolamins of Triticeae. Avenin proteins presented a lower proline content than that of wheat gliadin; this may contribute to the low toxicity shown by oat avenins. The expression of avenin genes throughout the development stages has shown a pattern similar to that of prolamins of wheat and barley. RP-HPLC chromatograms showed protein peaks in the alcohol-soluble and reduced-soluble fractions. Therefore, oat grains had both monomeric and polymeric avenins, termed in this paper gliadin- and glutenin-like avenins. We found a direct correlation between the immunogenicity of the different oat varieties and the presence of the specific peptides with a higher/lower potential immunotoxicity. The specific peptides from the oat variety with the highest toxicity have shown a higher potential immunotoxicity. These results suggest that there is wide range of variation of potential immunotoxicity of oat cultivars that could be due to differences in the degree of immunogenicity in their sequences. © 2012 Real et al.<br />This work was supported by the Spanish Ministerio de Economía y Competitividad (MINECO,Programa INNPACTO, IPT-2011-1321-010000) and the European Regional Development Fund (FEDER). FP is supported by a Ramón y Cajal research contract from the MINECO (RYC-2010-07345).

Subjects

Subjects :
Proteomics
Male
Avena
Agricultural Biotechnology
Prolamin
Diseases::Nutritional and Metabolic Diseases::Metabolic Diseases::Malabsorption Syndromes::Celiac Disease [Medical Subject Headings]
Gliadin
RNA interference
Gene Expression Regulation, Plant
immune system diseases
Cloning, Molecular
Child
Triticeae
skin and connective tissue diseases
Avenin
chemistry.chemical_classification
Multidisciplinary
biology
Genetically Modified Organisms
Technology, Industry, Agriculture::Food and Beverages::Food::Cereals::Avena sativa [Medical Subject Headings]
Immunogenicity
Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Plant Proteins::Seed Storage Proteins::Prolamins [Medical Subject Headings]
Enfermedad Celíaca
Prolaminas
food and beverages
Agriculture
Complementary DNA
Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Plant Proteins::Seed Storage Proteins::Prolamins::Glutens [Medical Subject Headings]
Biochemistry
Child, Preschool
Wheat
Toxicity
Medicine
Female
Genetic Engineering
Research Article
Biotechnology
musculoskeletal diseases
Glutens
Science
Molecular Sequence Data
Cereals
Crops
Avena sativa
Structure-Activity Relationship
Species Specificity
Antigen
Genetics
otorhinolaryngologic diseases
Humans
Amino Acid Sequence
Proline
Protein Structure, Quaternary
Biology
Transgenic Plants
Gamma interferon
Dieta Sin Gluten
fungi
Infant
Glútenes
biology.organism_classification
Gluten
Peptide Fragments
Celiac Disease
stomatognathic diseases
chemistry
biology.protein
Analytical, Diagnostic and Therapeutic Techniques and Equipment::Therapeutics::Nutrition Therapy::Diet Therapy::Diet, Gluten-Free [Medical Subject Headings]
RNA
Plant Biotechnology
Gene expression
Protein Multimerization

Details

Database :
OpenAIRE
Journal :
idUS. Depósito de Investigación de la Universidad de Sevilla, instname, Digital.CSIC. Repositorio Institucional del CSIC, PLoS ONE, Vol 7, Iss 12, p e48365 (2012), PLoS ONE
Accession number :
edsair.doi.dedup.....01aed59dccecedb7b0ebf5328bdb907f