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Purification and Characterization of DNA Polymerases from the Plasmacytoma MOPC 104E and Abelson Murine Leukemia Viruses
- Source :
- European Journal of Biochemistry. 85:571-579
- Publication Year :
- 1978
- Publisher :
- Wiley, 1978.
-
Abstract
- RNA-dependent DNA polymerases of intracisternal A particles from the mouse plasma cell tumor MOPC 104E and of Abelson murine leukemia virus (A-MuLV) were isolated from particle preparations by Nonidet P40 and ultrasonic treatment and purified by column chromatography on DEAE-cellulose and phosphocellulose, followed by centrifugation in linear sucrose gradients. Both DNA polymerases were very similar in their elution patterns from phospho and DEAE-cellulose, template specificities, requirements for optimum activity and inactivation by anti-(reverse transcriptase) antiserum. They are associated with ribonuclease H activity. For molecular weight determinations, antibody-precipitated enzymes were bound to staphylococcal-protein-A-Sepharose, solubilized and run on dodecylsulfate/polyacrylamide gels. Their apparent molecular weight was estimated to be 80000.
- Subjects :
- chemistry.chemical_classification
Antiserum
biology
Abelson murine leukemia virus
DNA polymerase
DNA-Directed DNA Polymerase
biology.organism_classification
Biochemistry
Molecular biology
Reverse transcriptase
Cell Line
Leukemia Virus, Murine
Molecular Weight
Kinetics
Mice
Enzyme
chemistry
Cell culture
biology.protein
Animals
Intracisternal A-Particle
Centrifugation
Plasmacytoma
Subjects
Details
- ISSN :
- 14321033 and 00142956
- Volume :
- 85
- Database :
- OpenAIRE
- Journal :
- European Journal of Biochemistry
- Accession number :
- edsair.doi.dedup.....01a0a95e5e6d3bb7cc587fdfe37a1d59