Back to Search
Start Over
Hydrocortisone-induced inhibition of reactive oxygen species by polymorphonuclear neutrophils
- Source :
- Critical care medicine. 27(11)
- Publication Year :
- 1999
-
Abstract
- To determine whether hydrocortisone given intravenously inhibits reactive oxygen species (ROS) generation by polymorphonuclear neutrophils (PMNLs) in vivo and, if so, to describe the pharmacodynamics of this effect.A prospective, open label study in normal subjects.A clinical research unit of a tertiary referral center for diabetes and endocrinology.Eight normal subjects (age range, 2450 yrs).An indwelling cannula was inserted into the antecubital vein. Sequential blood samples were obtained from the cannula just before, and after, the intravenous injection of hydrocortisone (100 mg) at 1, 2, 4, 8, and 24 hrs.ROS generation by PMNLs and mononuclear cells (MNCs) was assayed as previously observed in a chemiluminometer. ROS generation by PMNLs and MNCs was inhibited by hydrocortisone at 1 hr; this effect peaked at 2 hrs and began to recover by 4 hrs; ROS generation had recovered to the baseline by 24 hrs. Although the pharmacodynamic effect of hydrocortisone on PMNLs and MNCs was similar, the peak inhibition was significantly greater for PMNLs (26% of basal vs. 43% of basal, p.02) than MNCs.There is a marked, consistent, inhibition of ROS generation by PMNLs, which parallels that of MNCs after intravenous hydrocortisone. The pharmacodynamics of this effect are consistent with our current clinical practices.
- Subjects :
- Adult
medicine.medical_specialty
Hydrocortisone
medicine.drug_class
Neutrophils
Neutrophile
Anti-Inflammatory Agents
Critical Care and Intensive Care Medicine
Peripheral blood mononuclear cell
Blood cell
In vivo
Reference Values
Internal medicine
medicine
Humans
Prospective Studies
chemistry.chemical_classification
Reactive oxygen species
business.industry
Biological activity
Middle Aged
Endocrinology
medicine.anatomical_structure
chemistry
Pharmacodynamics
Injections, Intravenous
Leukocytes, Mononuclear
Corticosteroid
business
Reactive Oxygen Species
Subjects
Details
- ISSN :
- 00903493
- Volume :
- 27
- Issue :
- 11
- Database :
- OpenAIRE
- Journal :
- Critical care medicine
- Accession number :
- edsair.doi.dedup.....019cd5f67f86014c1943fed7b5b6c66a