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High-throughput hydrophilic interaction chromatography coupled to tandem mass spectrometry for the optimized quantification of the anti-Gram-negatives antibiotic colistin A/B and its pro-drug colistimethate
- Source :
- Journal of Chromatography A, Journal of Chromatography A, Elsevier, 2014, 1369, pp.52-63. ⟨10.1016/j.chroma.2014.09.063⟩
- Publication Year :
- 2014
- Publisher :
- HAL CCSD, 2014.
-
Abstract
- Colistin is a last resort's antibacterial treatment in critically ill patients with multi-drug resistant Gram-negative infections. As appropriate colistin exposure is the key for maximizing efficacy while minimizing toxicity, individualized dosing optimization guided by therapeutic drug monitoring is a top clinical priority. Objective of the present work was to develop a rapid and robust HPLC-MS/MS assay for quantification of colistin plasma concentrations. This novel methodology validated according to international standards simultaneously quantifies the microbiologically active compounds colistin A and B, plus the pro-drug colistin methanesulfonate (colistimethate, CMS). 96-well micro-Elution SPE on Oasis Hydrophilic-Lipophilic-Balanced (HLB) followed by direct analysis by Hydrophilic Interaction Liquid Chromatography (HILIC) with Ethylene Bridged Hybrid--BEH--Amide phase column coupled to tandem mass spectrometry allows a high-throughput with no significant matrix effect. The technique is highly sensitive (limit of quantification 0.014 and 0.006 μg/mL for colistin A and B), precise (intra-/inter-assay CV 0.6-8.4%) and accurate (intra-/inter-assay deviation from nominal concentrations -4.4 to +6.3%) over the clinically relevant analytical range 0.05-20 μg/mL. Colistin A and B in plasma and whole blood samples are reliably quantified over 48 h at room temperature and at +4°C (6% deviation from nominal values) and after three freeze-thaw cycles. Colistimethate acidic hydrolysis (1M H2SO4) to colistin A and B in plasma was completed in vitro after 15 min of sonication while the pro-drug hydrolyzed spontaneously in plasma ex vivo after 4 h at room temperature: this information is of utmost importance for interpretation of analytical results. Quantification is precise and accurate when using serum, citrated or EDTA plasma as biological matrix, while use of heparin plasma is not appropriate. This new analytical technique providing optimized quantification in real-life conditions of the microbiologically active compounds colistin A and B offers a highly efficient tool for routine therapeutic drug monitoring aimed at individualizing drug dosing against life-threatening infections.
- Subjects :
- 1303 Biochemistry
[SDV]Life Sciences [q-bio]
610 Medicine & health
Biochemistry
High-performance liquid chromatography
Analytical Chemistry
Matrix (chemical analysis)
Tandem Mass Spectrometry
medicine
Humans
Prodrugs
Solid phase extraction
Chromatography, High Pressure Liquid
ComputingMilieux_MISCELLANEOUS
Polymyxin B
Detection limit
1602 Analytical Chemistry
Chromatography
medicine.diagnostic_test
Colistin
Chemistry
Hydrophilic interaction chromatography
Solid Phase Extraction
Organic Chemistry
General Medicine
[SDV.SP]Life Sciences [q-bio]/Pharmaceutical sciences
3. Good health
10199 Clinic for Clinical Pharmacology and Toxicology
Therapeutic drug monitoring
Calibration
Drug Monitoring
Hydrophobic and Hydrophilic Interactions
1605 Organic Chemistry
medicine.drug
Colistimethate
Subjects
Details
- Language :
- English
- ISSN :
- 00219673 and 18733778
- Database :
- OpenAIRE
- Journal :
- Journal of Chromatography A, Journal of Chromatography A, Elsevier, 2014, 1369, pp.52-63. ⟨10.1016/j.chroma.2014.09.063⟩
- Accession number :
- edsair.doi.dedup.....0183dae6e88eda21ef4c70d4c926e5fe