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Serial Noninvasive In Vivo Positron Emission Tomographic Tracking of Percutaneously Intramyocardially Injected Autologous Porcine Mesenchymal Stem Cells Modified for Transgene Reporter Gene Expression
- Source :
- Circulation: Cardiovascular Imaging. 1:94-103
- Publication Year :
- 2008
- Publisher :
- Ovid Technologies (Wolters Kluwer Health), 2008.
-
Abstract
- Background— Porcine bone marrow-derived mesenchymal stem cells (MSCs) were stably transfected with a lentiviral vector for transgene expression of the trifusion protein renilla luciferase, red fluorescent protein and herpes simplex truncated thymidine kinase (LV-RL-RFP-tTK; positron emission tomography [PET] reporter gene) for in vivo noninvasive tracking of the intramyocardially delivered MSC fate. Methods and Results— A closed-chest, reperfused myocardial infarction was created in farm pigs. Sixteen days after myocardial infarction, LV-RL-RFP-tTK-MSCs were injected intramyocardially using electromechanical mapping guidance in the infarct border zone (n=7). PET-computed tomographic metabolic and perfusion imaging was performed after an intravenous injection of 10 mCi [18F]-FHBG and 13N-ammonia PET at 30�2 hours and 7 days after LV-RL-RFP-tTK-MSC treatment. Fusion imaging of the [18F]-FHBG PET-computed tomography with MRI was used to determine the myocardial location of the injected LV-RL-RFP-tTK-MSCs. Seven days after injections, [18F]-FHBG PET showed a decreased cardiac uptake with a mild increased pericardial and pleura uptake in the treated animals, which was confirmed by the measurement of luciferase activity. At 10 days, infarct size by MRI in the LV-RL-RFP-tTK-MSC-treated animals was smaller than controls (n=7) (23.3�1.5% versus 30.2�3.5%, P Conclusions— Reporter gene imaging enables the tracking of the persistence of viable LV-RL-RFP-tTK-MSC in the peri-infarcted porcine myocardium at 10 days after delivery using clinical PET scanners.
- Subjects :
- Fluorine Radioisotopes
Pathology
medicine.medical_specialty
Guanine
Sus scrofa
Myocardial Infarction
Gene Expression
Infarction
Perfusion scanning
Mesenchymal Stem Cell Transplantation
Transfection
Article
Injections
Green fluorescent protein
Genes, Reporter
In vivo
medicine
Animals
Radiology, Nuclear Medicine and imaging
Transgenes
Reporter gene
medicine.diagnostic_test
business.industry
Myocardium
Mesenchymal stem cell
Heart
medicine.disease
Magnetic Resonance Imaging
Positron emission tomography
Thymidine kinase
Positron-Emission Tomography
Cardiology and Cardiovascular Medicine
Nuclear medicine
business
Subjects
Details
- ISSN :
- 19420080 and 19419651
- Volume :
- 1
- Database :
- OpenAIRE
- Journal :
- Circulation: Cardiovascular Imaging
- Accession number :
- edsair.doi.dedup.....017c9c1fa05a4dbddbd1933293fe547f