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Evidence for an alternate splicing in the thyroperoxidase messenger from patients with Graves' disease
- Source :
- Biochemical and Biophysical Research Communications. 170:735-741
- Publication Year :
- 1990
- Publisher :
- Elsevier BV, 1990.
-
Abstract
- An initial lambda gt 11 cDNA library constructed from a human Graves' patient thyroid was screened with an immunopurified rabbit anti-human thyroperoxidase (hTPO) polyclonal antibody. A 869 bp clone was obtained. It presents a 130 bp deletion as compared to the published sequence and a 77 bp insertion in the 3' non-coding region. Screening of a pUC cDNA library from another Graves' patient thyroid exhibited the same 130 bp deletion in two other cDNA clones. PCR analysis of mRNA transcripts confirmed the presence of the two messengers in two other Graves' thyroid tissues. In all the cases, this new spliced mRNA species represents between 40% and 50% of the total hTPO mRNAs. With respect to the structure of the hTPO gene, the present deletion suggests an alternate splicing of exon 16. The juxtaposition of exon 17 to exon 15 encoding the transmembrane domain leads to a shift in the reading frame. By the use of a different stop codon, the spliced mRNA generates a modified 56 - COOH terminal aminoacids (aa) sequence.
- Subjects :
- RNA Splicing
Graves' disease
Molecular Sequence Data
Biophysics
Biology
Iodide Peroxidase
Polymerase Chain Reaction
Biochemistry
Exon
Thyroid peroxidase
Complementary DNA
medicine
Humans
Amino Acid Sequence
RNA, Messenger
Molecular Biology
Genetics
Genomic Library
Base Sequence
cDNA library
Alternative splicing
DNA
Exons
Cell Biology
medicine.disease
Molecular biology
Graves Disease
Stop codon
RNA splicing
biology.protein
Poly A
Subjects
Details
- ISSN :
- 0006291X
- Volume :
- 170
- Database :
- OpenAIRE
- Journal :
- Biochemical and Biophysical Research Communications
- Accession number :
- edsair.doi.dedup.....014306f4a622e07193c310b1750b8a00