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Simultaneous multi-color optical sectioning fluorescence microscopy with wavelength-coded volume holographic gratings
- Source :
- Optics express. 28(25)
- Publication Year :
- 2020
-
Abstract
- Optical sectioning fluorescence microscopy provides high contrast images of volumetric samples and has been widely used for many biological applications. However, simultaneously acquiring multi-color fluorescence images require additional optical elements and devices, which are bulky, wavelength specific, and not cost-effective. In this paper, wavelength-coded volume holographic gratings (WC-VHGs) based optical sectioning fluorescence microscopy is proposed to simultaneously offer multi-color fluorescence images with fine out-of-focus background rejection. Due to wavelength degeneracy, multiplexed WC-VHGs are capable of acquiring multi-wavelength fluorescence images in a single shot, and displaying the laterally separated multi-wavelength images onto CCD. In our system optical sectioning capability is achieved through speckle illumination and HiLo imaging method. To demonstrate imaging characteristics of our system, dual-wavelength fluorescence images of both standard fluorescent microspheres and ex vivo mT/mG mice cardiac tissue are presented. Current results may find important applications in hyperspectral imaging for biomedical research.
- Subjects :
- Materials science
genetic structures
Optical sectioning
Optical Phenomena
Holography
Image processing
02 engineering and technology
01 natural sciences
Fluorescence
law.invention
010309 optics
Speckle pattern
Mice
Optics
law
0103 physical sciences
Fluorescence microscope
Image Processing, Computer-Assisted
Animals
Lighting
business.industry
Optical Imaging
Hyperspectral imaging
Heart
021001 nanoscience & nanotechnology
Atomic and Molecular Physics, and Optics
Microspheres
Wavelength
Microscopy, Fluorescence
sense organs
0210 nano-technology
business
Subjects
Details
- ISSN :
- 10944087
- Volume :
- 28
- Issue :
- 25
- Database :
- OpenAIRE
- Journal :
- Optics express
- Accession number :
- edsair.doi.dedup.....01243aa63ad6d9bf275c08b21790483e