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Simultaneous multi-color optical sectioning fluorescence microscopy with wavelength-coded volume holographic gratings

Authors :
Yuan Luo
J. Andrew Yeh
Yi-You Huang
Yu-Hsin Chia
Source :
Optics express. 28(25)
Publication Year :
2020

Abstract

Optical sectioning fluorescence microscopy provides high contrast images of volumetric samples and has been widely used for many biological applications. However, simultaneously acquiring multi-color fluorescence images require additional optical elements and devices, which are bulky, wavelength specific, and not cost-effective. In this paper, wavelength-coded volume holographic gratings (WC-VHGs) based optical sectioning fluorescence microscopy is proposed to simultaneously offer multi-color fluorescence images with fine out-of-focus background rejection. Due to wavelength degeneracy, multiplexed WC-VHGs are capable of acquiring multi-wavelength fluorescence images in a single shot, and displaying the laterally separated multi-wavelength images onto CCD. In our system optical sectioning capability is achieved through speckle illumination and HiLo imaging method. To demonstrate imaging characteristics of our system, dual-wavelength fluorescence images of both standard fluorescent microspheres and ex vivo mT/mG mice cardiac tissue are presented. Current results may find important applications in hyperspectral imaging for biomedical research.

Details

ISSN :
10944087
Volume :
28
Issue :
25
Database :
OpenAIRE
Journal :
Optics express
Accession number :
edsair.doi.dedup.....01243aa63ad6d9bf275c08b21790483e