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Untargeted metabolite profiling for koji-fermentative bioprocess unravels the effects of varying substrate types and microbial inocula
- Source :
- Food chemistry. 266
- Publication Year :
- 2018
-
Abstract
- Untargeted metabolomics unraveled the effects of varying substrates (soybean, wheat, and rice) and inocula (Aspergillus oryzae and Bacillus amyloliquefaciens) on metabolite compositions of koji, a starter ingredient in various Asian fermented foods. Multivariate analyses of the hyphenated mass spectrometry datasets for different koji extracts highlighted 61 significantly discriminant primary metabolites (sugars and sugar alcohols, organic acids, amino acids, fatty acids, nucleosides, phenolic acids, and vitamins) according to varying substrates and inocula combinations. However, 59 significantly discriminant secondary metabolites were evident for koji-types with varying substrates only, viz., soybean (flavonoids, soyasaponins, and lysophospholipids), wheat (flavones and lysophospholipids), and rice (flavonoids, fatty acids derivatives, and lysophospholipids). Independently, the substrates influenced primary metabolite compositions in koji (soybean > wheat, rice). The inocula choice of A. oryzae engendered higher carbohydrates, organic acids, and lipid derivative levels commensurate with high α-amylase and β-glucosidase activities, while B. amyloliquefaciens affected higher amino acids levels, in respective koji types.
- Subjects :
- 0106 biological sciences
0301 basic medicine
Bacillus amyloliquefaciens
Metabolite
Aspergillus oryzae
Carbohydrates
Lysophospholipids
01 natural sciences
Flavones
Gas Chromatography-Mass Spectrometry
Analytical Chemistry
03 medical and health sciences
chemistry.chemical_compound
010608 biotechnology
Hydroxybenzoates
Metabolomics
Food science
Amino Acids
Sugar
Chromatography, High Pressure Liquid
Triticum
chemistry.chemical_classification
Flavonoids
Principal Component Analysis
biology
Chemistry
beta-Glucosidase
food and beverages
Primary metabolite
Oryza
General Medicine
Vitamins
Hydrogen-Ion Concentration
biology.organism_classification
Amino acid
030104 developmental biology
Soybeans
alpha-Amylases
Food Science
Subjects
Details
- ISSN :
- 18737072
- Volume :
- 266
- Database :
- OpenAIRE
- Journal :
- Food chemistry
- Accession number :
- edsair.doi.dedup.....011e7fb3d7ac430237124e539fdc8724