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UBF levels determine the number of active ribosomal RNA genes in mammals
- Source :
- The Journal of Cell Biology
- Publication Year :
- 2008
-
Abstract
- In mammals, the mechanisms regulating the number of active copies of the ∼200 ribosomal RNA (rRNA) genes transcribed by RNA polymerase I are unclear. We demonstrate that depletion of the transcription factor upstream binding factor (UBF) leads to the stable and reversible methylation-independent silencing of rRNA genes by promoting histone H1–induced assembly of transcriptionally inactive chromatin. Chromatin remodeling is abrogated by the mutation of an extracellular signal-regulated kinase site within the high mobility group box 1 domain of UBF1, which is required for its ability to bend and loop DNA in vitro. Surprisingly, rRNA gene silencing does not reduce net rRNA synthesis as transcription from remaining active genes is increased. We also show that the active rRNA gene pool is not static but decreases during differentiation, correlating with diminished UBF expression. Thus, UBF1 levels regulate active rRNA gene chromatin during growth and differentiation.
- Subjects :
- Transcription, Genetic
5.8S ribosomal RNA
Fluorescent Antibody Technique
Biology
DNA, Ribosomal
Chromatin remodeling
Article
Histones
03 medical and health sciences
Mice
0302 clinical medicine
23S ribosomal RNA
Transcription (biology)
RNA Polymerase I
RNA polymerase I
Animals
Protein Isoforms
Gene Silencing
Transcription factor
Research Articles
030304 developmental biology
0303 health sciences
Models, Genetic
Genes, rRNA
Cell Biology
Ribosomal RNA
DNA Methylation
Chromatin Assembly and Disassembly
Molecular biology
Chromatin
030220 oncology & carcinogenesis
HMG-Box Domains
NIH 3T3 Cells
Pol1 Transcription Initiation Complex Proteins
Subjects
Details
- ISSN :
- 15408140
- Volume :
- 183
- Issue :
- 7
- Database :
- OpenAIRE
- Journal :
- The Journal of cell biology
- Accession number :
- edsair.doi.dedup.....010fb93b182a661701d35d7c8525a7c7