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Pressure Effect on the Membrane Action of a Nerve-Blocking Spin Label
- Source :
- Proceedings of the National Academy of Sciences. 70:3179-3183
- Publication Year :
- 1973
- Publisher :
- Proceedings of the National Academy of Sciences, 1973.
-
Abstract
- A reversible nerve-blocking spin label, 2,2,6,6-tetramethylpiperidine-1-oxyl (TEMPO) was used to study the nature of anesthetic-binding sites in nerve membranes as a function of pressure. The nerve-blocking effect of TEMPO is enhanced under pressure. At atmospheric pressure, TEMPO blocks nerve conduction by solubilizing in the apolar region of the nerve membrane. However, the nerve-conduction-block by TEMPO at 150 atm of helium was related to the binding of TEMPO to a pressure-induced high-affinity polar site in the nerve membrane. The new TEMPO-binding site could not be detected in lipid model membranes and, thus, the involvement of membrane protein in the new site was inferred. Pressure may induce a nerve membrane conformation change in the presence of TEMPO. The observation that under different pressure, a single anesthetic, i.e., TEMPO, was capable of blocking nerve conduction by binding to two different sites within the nerve membrane, supports the view that there are multiple anesthetic receptor sites, which differ in chemical composition and location within the nerve membrane. These sites, when occupied by different classes of anesthetics, produce the general phenomenon of nerve-conduction block. The enhancement of nerve-conduction block by pressure may be due to the increased concentration of TEMPO in the new site in the nerve membrane under pressure.
- Subjects :
- Receptors, Drug
medicine.medical_treatment
Molecular Conformation
Neural Conduction
Action Potentials
In Vitro Techniques
Helium
behavioral disciplines and activities
Cyclic N-Oxides
Cell membrane
Structure-Activity Relationship
Piperidines
medicine
Animals
Spin label
Phrenic nerve
Neurons
Multidisciplinary
Chemistry
Cell Membrane
Electron Spin Resonance Spectroscopy
Membranes, Artificial
Nerve Block
Sciatic Nerve
humanities
Rats
Phrenic Nerve
Atmospheric Pressure
Membrane
medicine.anatomical_structure
Solubility
Biochemistry
Membrane protein
Biophysics
Nerve block
Spin Labels
Biological Sciences: Biochemistry
Sciatic nerve
Anura
Subjects
Details
- ISSN :
- 10916490 and 00278424
- Volume :
- 70
- Database :
- OpenAIRE
- Journal :
- Proceedings of the National Academy of Sciences
- Accession number :
- edsair.doi.dedup.....0102462ce6cd9f40e6c9cab9408bb7ff