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Molecular characterization, isolation, pathology and pathotyping of peafowl (Pavo cristatus) origin Newcastle disease virus isolates recovered from disease outbreaks in three states of India
- Source :
- Avian pathology : journal of the W.V.P.A. 45(6)
- Publication Year :
- 2016
-
Abstract
- Disease outbreak investigations were carried out in three states of Northern India namely Haryana (Rewari), Uttar Pradesh (Noida) and Delhi, where a total of 110 Indian peafowls (Pavo cristatus) showed sudden onset of nervous signs and died within a period of two weeks during June, 2012. The F (fusion) gene-based RT-PCR detection of Newcastle disease virus (NDV) in affected tissues confirmed the presence of the virus. Three NDV isolates were selected (one from each area under investigation) and further characterized. They were found to be of virulent pathotype (velogenic NDV) based on both pathogenicity assays (MDT, ICPI and IVPI) and partial F gene sequence analysis. Additionally, the phylogenetic analysis revealed that the isolates belonged to the genotype VIIi and XIII of class II avian Paramyxovirus serotype1 (APMV-1) and related closely to new emerging sub-genotypes. This is the first report regarding the presence of the fifth panzootic vNDV genotype VIIi from India. In this scenario, extensive epidemiological studies are suggested for surveillance of NDV genotypes in wild birds and poultry flocks of the country along with adopting suitable prevention and control measures.
- Subjects :
- 0301 basic medicine
Pathology
medicine.medical_specialty
Veterinary medicine
Genotype
Sequence analysis
Newcastle Disease
Newcastle disease virus
Virulence
India
Chick Embryo
Newcastle disease
Virus
Disease Outbreaks
03 medical and health sciences
Feces
Food Animals
medicine
Animals
Galliformes
Panzootic
Phylogeny
General Immunology and Microbiology
biology
Phylogenetic tree
Outbreak
Sequence Analysis, DNA
biology.organism_classification
Virology
Specific Pathogen-Free Organisms
030104 developmental biology
Animal Science and Zoology
Viral Fusion Proteins
Subjects
Details
- ISSN :
- 14653338
- Volume :
- 45
- Issue :
- 6
- Database :
- OpenAIRE
- Journal :
- Avian pathology : journal of the W.V.P.A
- Accession number :
- edsair.doi.dedup.....00fe17ee904baf0a7823ccf66a05dfaf