Back to Search
Start Over
Graphitic Carbon Nitride as an Amplification Platform on an Electrochemical Paper-Based Device for the Detection of Norovirus-Specific DNA
- Source :
- Sensors, Vol 20, Iss 2070, p 2070 (2020), Sensors (Basel, Switzerland), Sensors, Volume 20, Issue 7, Sensors (Basel) 20 (2020). doi:10.3390/s20072070, info:cnr-pdr/source/autori:Rana A.; Killa M.; Yadav N.; Mishra A.; Mathur A.; Kumar A.; Khanuja M.; Narang J.; Pilloton R./titolo:Graphitic carbon nitride as an amplification platform on an electrochemical paper-based device for the detection of norovirus-specific DNA/doi:10.3390%2Fs20072070/rivista:Sensors (Basel)/anno:2020/pagina_da:/pagina_a:/intervallo_pagine:/volume:20
- Publication Year :
- 2020
- Publisher :
- MDPI AG, 2020.
-
Abstract
- Norovirus is one of the leading causes of gastroenteritis, acute vomiting, intense diarrhoea, acute pain in the stomach, high fever, headaches, and body pain. Conventional methods of detection gave us very promising results but had disadvantages such as low sensitivity, cost ineffectiveness, reduced specificity and selectivity, etc. Therefore, biosensors can be a viable alternative device which can overcome all setbacks associated with the conventional method. An electrochemical sensor based on oxidized graphitic carbon nitride (Ox-g-C3N4) modified electrochemical paper-based analytical device (ePAD) was fabricated for the detection of norovirus DNA. The synthesized Ox-g-C3N4 nanosheets were characterized by field emission scanning electron microscopy (FESEM), X-ray Diffraction (XRD), UV-Vis spectroscopy and X-Ray Photoelectron Spectroscopy. The capture probe DNA (PDNA) modified electrodes were characterized by cyclic voltammetry (CV) and differential pulse voltammetry (DPV). These two characterization techniques were also employed to find the optimal scan rate, response time and temperature of the fabricated sensor. The fabricated biosensor showed a limit of detection (LOD) of 100 fM. Furthermore, the specificity of the reported biosensor was affirmed by testing the response of capture probe DNA with oxidized graphitic carbon nitride (PDNA/Ox-g-C3N4) modified ePAD on the introduction of a non-complimentary DNA. The fabricated ePAD sensor is easy to fabricate, cost effective and specific, and requires a minimum analysis time of 5 s.
- Subjects :
- Paper
Materials science
Immobilized Nucleic Acids
norovirus
Amplification Platform
Nanotechnology
02 engineering and technology
Biosensing Techniques
lcsh:Chemical technology
01 natural sciences
Biochemistry
Article
Analytical Chemistry
chemistry.chemical_compound
Limit of Detection
lcsh:TP1-1185
Electrical and Electronic Engineering
Nitrogen Compounds
Instrumentation
Electrodes
Detection limit
Horizontal scan rate
010401 analytical chemistry
Graphitic carbon nitride
technology, industry, and agriculture
Nucleic Acid Hybridization
Electrochemical Paper-Based Biosensor
DNA
Electrochemical Techniques
021001 nanoscience & nanotechnology
paper-based analytical device
oxidized graphitic carbon nitride
Atomic and Molecular Physics, and Optics
0104 chemical sciences
Electrochemical gas sensor
Nanostructures
Graphitic Carbon Nitride
chemistry
Electrode
RNA, Viral
methylene blue
Graphite
Differential pulse voltammetry
Cyclic voltammetry
0210 nano-technology
DNA Probes
Biosensor
genosensor
Subjects
Details
- Language :
- English
- ISSN :
- 14248220
- Volume :
- 20
- Issue :
- 2070
- Database :
- OpenAIRE
- Journal :
- Sensors
- Accession number :
- edsair.doi.dedup.....00fd8820d5814d2e3de483365ce9442c
- Full Text :
- https://doi.org/10.3390/s20072070