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An echistatin C-terminal peptide activates GPIIbIIIa binding to fibrinogen, fibronectin, vitronectin and collagen type I and type IV
An echistatin C-terminal peptide activates GPIIbIIIa binding to fibrinogen, fibronectin, vitronectin and collagen type I and type IV
- Source :
- The Biochemical journal. 293
- Publication Year :
- 1993
-
Abstract
- Integrin binding to proteins often involves recognition of domains containing the arginine-glycine-aspartate (RGD) motif. Different binding affinities and specificities of the integrin-ligand protein interactions involve additional protein domains. The n.m.r. structure of the snake-venom protein echistatin suggested that the C-terminal portion of the molecule might be important, in addition to the RGD domain, in binding to the integrin glycoprotein IIbIIIa (GPIIbIIIa) [Saudek, Atkinson and Pelton (1991) Biochem. 30, 7369-7372]. The synthetic C-terminal peptide, echistatin-(40-49), PRNPHKGPAT, (1) inhibited binding of GPIIbIIIa to immobilized echistatin (IC50 3-6 mM), but did not inhibit binding of GPIIbIIIa to immobilized fibrinogen (up to 5 mM peptide), (2) activated GPIIbIIIa binding to fibronectin and vitronectin, usual ligands for the activated integrin, (3) activated binding of GPIIbIIIa to collagen type I and type IV, proteins not usually regarded as ligands for the integrin, and (4) stimulated 125I-fibrinogen binding by human platelets. These findings argue for an interaction of this non-RGD domain in echistatin with GPIIbIIIa, leading to activation of the integrin and extension of the ligand specificity to include immobilized collagen.
- Subjects :
- Blood Platelets
Protein domain
Integrin
Molecular Sequence Data
Peptide
Enzyme-Linked Immunosorbent Assay
Platelet Membrane Glycoproteins
Viper Venoms
Biochemistry
Humans
Amino Acid Sequence
Vitronectin
Molecular Biology
Integrin binding
Glycoproteins
chemistry.chemical_classification
Binding Sites
biology
Binding protein
Fibrinogen
Cell Biology
Fibronectins
Fibronectin
chemistry
Biophysics
biology.protein
Intercellular Signaling Peptides and Proteins
Collagen
Glycoprotein
Peptides
Platelet Aggregation Inhibitors
Research Article
Subjects
Details
- ISSN :
- 02646021
- Volume :
- 293
- Database :
- OpenAIRE
- Journal :
- The Biochemical journal
- Accession number :
- edsair.doi.dedup.....00f08766a54fb03969f2c4628955eb04