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Transcriptional Induction of Metallothionein by Tris(pentafluorophenyl)stibane in Cultured Bovine Aortic Endothelial Cells
- Source :
- International Journal of Molecular Sciences, Vol 17, Iss 9, p 1381 (2016), International Journal of Molecular Sciences; Volume 17; Issue 9; Pages: 1381, International Journal of Molecular Sciences
- Publication Year :
- 2016
- Publisher :
- MDPI AG, 2016.
-
Abstract
- Vascular endothelial cells cover the luminal surface of blood vessels and contribute to the prevention of vascular disorders such as atherosclerosis. Metallothionein (MT) is a low molecular weight, cysteine-rich, metal-binding, inducible protein, which protects cells from the toxicity of heavy metals and active oxygen species. Endothelial MT is not induced by inorganic zinc. Adequate tools are required to investigate the mechanisms underlying endothelial MT induction. In the present study, we found that an organoantimony compound, tris(pentafluorophenyl)stibane, induces gene expression of MT-1A and MT-2A, which are subisoforms of MT in bovine aortic endothelial cells. The data reveal that MT-1A is induced by activation of both the MTF-1-MRE and Nrf2-ARE pathways, whereas MT-2A expression requires only activation of the MTF-1-MRE pathway. The present data suggest that the original role of MT-1 is to protect cells from heavy metal toxicity and oxidative stress in the biological defense system, while that of MT-2 is to regulate intracellular zinc metabolism.
- Subjects :
- 0301 basic medicine
Transcription, Genetic
Metal toxicity
medicine.disease_cause
lcsh:Chemistry
chemistry.chemical_compound
0302 clinical medicine
Gene expression
Hydrocarbons, Chlorinated
Protein Isoforms
Metallothionein
lcsh:QH301-705.5
Aorta
Spectroscopy
General Medicine
Computer Science Applications
Cell biology
DNA-Binding Proteins
Biochemistry
030220 oncology & carcinogenesis
Toxicity
vascular endothelial cell
Tris
NF-E2-Related Factor 2
Glutamate-Cysteine Ligase
Biology
Article
Catalysis
Inorganic Chemistry
03 medical and health sciences
bio-organometallics
metallothionein
organic–inorganic hybrid molecule
organoantimony compound
medicine
Animals
Physical and Theoretical Chemistry
Molecular Biology
Transcription factor
Organic Chemistry
Endothelial Cells
Metabolism
030104 developmental biology
chemistry
lcsh:Biology (General)
lcsh:QD1-999
Cattle
Heme Oxygenase-1
Oxidative stress
Transcription Factors
Subjects
Details
- Language :
- English
- ISSN :
- 14220067
- Volume :
- 17
- Issue :
- 9
- Database :
- OpenAIRE
- Journal :
- International Journal of Molecular Sciences
- Accession number :
- edsair.doi.dedup.....00ecca70774cae74cff4b2a98a36eacd