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Molecular species delimitation of shrub frogs of the genus Pseudophilautus (Anura, Rhacophoridae)
- Source :
- PLoS ONE, PLoS ONE, Vol 16, Iss 10, p e0258594 (2021)
- Publication Year :
- 2021
- Publisher :
- Public Library of Science, 2021.
-
Abstract
- Sri Lanka is an amphibian hotspot of global significance. Its anuran fauna is dominated by the shrub frogs of the genusPseudophilautus. Except for one small clade of four species in Peninsular India, these cool-wet adapted frogs, numbering some 59 extant species, are distributed mainly across the montane and lowland rain forests of the island. With species described primarily by morphological means, the diversification has never yet been subjected to a molecular species delimitation analysis, a procedure now routinely applied in taxonomy. Here we test the species boundaries ofPseudophilautusin the context of the phylogenetic species concept (PSC). We use all the putative species for which credible molecular data are available (nDNA–Rag-1; mt-DNA– 12S rRNA, 16S rRNA) to build a well resolved phylogeny, which is subjected to species delimitation analyses. The ABGD, bPTP, mPTP and bGMYC species delimitation methods applied to the 16S rRNA frog barcoding gene (for all species), 12S rRNA and Rag-1 nDNA groupedP.procaxandP.abundus;P.hallidayiandP.fergusonianus;P.reticulatusandP.pappilosus;P.pleurotaeniaandP.hoipolloi;P.hoffmaniandP.asankai;P.silvaticusandP.limbus;P.dilmahandP.hankeni;P.fulvusandP.silus.. Surprisingly, all analyses recovered 14 unidentified potential new species as well. The geophylogeny affirms a distribution across the island’s aseasonal ‘wet zone’ and its three principal hill ranges, suggestive of allopatric speciation playing a dominant role, especially between mountain masses. Among the species that are merged by the delimitation analyses, a pattern leading towards a model of parapatric speciation emerges–ongoing speciation in the presence of gene flow. This delimitation analysis reinforces the species hypotheses, paving the way to a reasonable understanding of Sri LankanPseudophilautus, enabling both deeper analyses and conservation efforts of this remarkable diversification.http://zoobank.org/urn:lsid:zoobank.org:pub:DA869B6B-870A-4ED3-BF5D-5AA3F69DDD27.
- Subjects :
- Species Delimitation
Speciation
Parapatric speciation
Biochemistry
Gene flow
Genus
RNA, Ribosomal, 16S
Databases, Genetic
Rhacophoridae
Pseudophilautus
Phylogeny
Data Management
Multidisciplinary
biology
Geography
Eukaryota
Phylogenetic Analysis
Nucleic acids
Phylogenetics
Phylogeography
Ribosomal RNA
Biogeography
Vertebrates
Medicine
Frogs
Taxonomy (biology)
Anura
Research Article
Cell biology
Computer and Information Sciences
Evolutionary Processes
Cellular structures and organelles
Science
Allopatric speciation
Zoology
India
Amphibian Proteins
Amphibians
Genetics
Animals
DNA Barcoding, Taxonomic
Evolutionary Systematics
Non-coding RNA
Taxonomy
Homeodomain Proteins
Evolutionary Biology
Population Biology
Ecology and Environmental Sciences
Organisms
Biology and Life Sciences
Sequence Analysis, DNA
biology.organism_classification
RNA, Ribosomal
Earth Sciences
RNA
Ribosomes
Population Genetics
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 16
- Issue :
- 10
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....00df657f99bfce577e8915110e660a95