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A Commercial Anti-TIF1γ ELISA Is Superior to Line and Dot Blot and Should Be Considered as Part of Routine Myositis-Specific Antibody Testing
- Source :
- Mulhearn, B, Li, D, McMorrow, F, Lu, H, McHugh, N J & Tansley, S L 2022, ' A Commercial Anti-TIF1γ ELISA Is Superior to Line and Dot Blot and Should Be Considered as Part of Routine Myositis-Specific Antibody Testing ', Frontiers in Immunology, vol. 13, 804037 . https://doi.org/10.3389/fimmu.2022.804037, Frontiers in Immunology, Vol 13 (2022)
- Publication Year :
- 2021
-
Abstract
- ObjectivesAnti-TIF1γ is an important autoantibody in the diagnosis of cancer-associated dermatomyositis and the most common autoantibody in juvenile onset dermatomyositis. Its reliable detection is important to instigate further investigations into underlying malignancy in adults. We previously showed that commercial assays using line and dot blots do not reliably detect anti-TIF1γ. We aimed to test a new commercial ELISA and compare with previously obtained protein immunoprecipitation.MethodsRadio-labelled immunoprecipitation had previously been used to determine the autoantibody status of patients with immune-mediated inflammatory myopathies and several healthy controls. ELISA was undertaken on healthy control and anti-TIF1γ sera and compared to previous immunoprecipitation data.ResultsA total of 110 serum samples were analysed: 42 myositis patients with anti- TIF1γ and 68 autoantibody negative healthy control sera. Anti-TIF1γ was detected by ELISA in 41 out of 42 of the anti-TIF1γ-positive samples by immunoprecipitation, and in none of the healthy controls, giving a sensitivity of 97.6% and specificity of 100%. The false negative rate was 2%.ConclusionELISA is an affordable and time-efficient method which is accurate in detecting anti-TIF1γ.
- Subjects :
- Immunoprecipitation/methods
autoantibodies
Immunology
Immunoblotting
Enzyme-Linked Immunosorbent Assay
Sensitivity and Specificity
Dermatomyositis
myositis - diagnosis
SDG 3 - Good Health and Well-being
cancer
Immunology and Allergy
Humans
Immunoprecipitation
Serologic Tests
TIF1γ
False Negative Reactions
Autoantibodies
Immunoblotting/methods
ELISA - enzyme-linked immunosorbent assay
Serologic Tests/methods
Enzyme-Linked Immunosorbent Assay/methods
Diagnostic Tests, Routine
dermatomysitis
RC581-607
Dermatomyositis/blood
Data Accuracy
Case-Control Studies
Diagnostic Tests, Routine/methods
Transcription Factors/immunology
Immunologic diseases. Allergy
Autoantibodies/blood
myositis
Transcription Factors
Subjects
Details
- ISSN :
- 16643224
- Volume :
- 13
- Database :
- OpenAIRE
- Journal :
- Frontiers in immunology
- Accession number :
- edsair.doi.dedup.....00dd1cbce928b211070eb55d26a55afc