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Expression of platelet-derived growth factor and activated receptor in clinical specimens of epithelial ovarian cancer and ovarian carcinoma cell lines
- Source :
- Gynecologic oncology. 93(1)
- Publication Year :
- 2003
-
Abstract
- Objectives . We determined the expression of platelet-derived growth factor (PDGF), PDGF-receptor (PDGF-R), and phosphorylated PDGF-R (p-PDGF-R) on tumor cells and tumor-associated endothelial cells in clinical specimens of human ovarian carcinoma and human ovarian cancer cells growing in culture and in the peritoneal cavity of nude mice. Methods . Ten specimens of high-grade serous ovarian carcinoma were analyzed using immunohistochemistry (IHC). IHC was used to detect ligand and receptor expression in the human ovarian cancer cells from Hey A8 and SKOV3ip1 growing in culture. Cells from these lines were also implanted orthotopically into the peritoneal cavity of nude mice. IHC was used to determine ligand and receptor expression in tumors that formed in the peritoneal cavity. Results . All 10 evaluable samples expressed both PDGF AA and BB on tumor cells. Tumor cells were positive for PDGF-Rα in 10/10 samples, PDGF-Rβ in 8/10 samples, p-PDGF-Rα in 6/10 samples, and p-PDGF-Rβ in 4/10 samples. p-PDGF-Rα was positive in 4/10 tumor-associated endothelial cell samples and p-PDGF-Rβ was positive in 3/10 samples. Human ovarian cancer cells expressed PDGF, PDGF-R, and p-PDGF-R when growing in culture or in the peritoneal cavity of nude mice. PDGF-R and p-PDGF-R were also present on tumor-associated endothelial cells as demonstrated by simultaneous staining with CD31 antibody. Conclusions . PDGF and the corresponding receptors were expressed in autochthonous human ovarian cancer lesions on both tumor cells and tumor-associated endothelial cells. The ligand and receptor were also present on Hey A8 and SKOV3ip1 human ovarian cancer cells growing in vitro and in the peritoneal cavity of nude mice.
- Subjects :
- CD31
Pathology
medicine.medical_specialty
Platelet-derived growth factor
Receptor, Platelet-Derived Growth Factor alpha
Receptor expression
Transplantation, Heterologous
Becaplermin
Mice, Nude
Biology
Receptor, Platelet-Derived Growth Factor beta
Peritoneal cavity
chemistry.chemical_compound
Mice
Ovarian carcinoma
Cell Line, Tumor
medicine
Animals
Humans
Phosphorylation
Ovarian Neoplasms
Platelet-Derived Growth Factor
Obstetrics and Gynecology
Proto-Oncogene Proteins c-sis
medicine.disease
Immunohistochemistry
Endothelial stem cell
medicine.anatomical_structure
Oncology
chemistry
Cell culture
Cancer research
Disease Progression
Female
Ovarian cancer
Neoplasm Transplantation
Subjects
Details
- ISSN :
- 00908258
- Volume :
- 93
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Gynecologic oncology
- Accession number :
- edsair.doi.dedup.....00cf50bea7247c1ee789995335a55421