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A PCR-free technology to detect and quantify microRNAs directly from human plasma

Authors :
Barbara López-Longarela
Francisco Javier López-Delgado
Antonio Marín-Romero
Rosario M. Sánchez-Martín
Juan J. Guardia-Monteagudo
Juan J. Díaz-Mochón
Salvatore Pernagallo
Agustín Robles-Remacho
Mario Antonio Fara
Mavys Tabraue-Chávez
Source :
The Analyst. 143:5676-5682
Publication Year :
2018
Publisher :
Royal Society of Chemistry (RSC), 2018.

Abstract

A novel sensitive, specific and rapid method for the detection and quantification of microRNAs without requiring extraction from their biological sources is now available using a novel chemical based, PCR-free technology for nucleic acid testing. In this study, we both demonstrate how this method can be used to profile miR-451a, an important miRNA in erythropoiesis, and compare with the gold standard RT-qPCR.

Details

ISSN :
13645528 and 00032654
Volume :
143
Database :
OpenAIRE
Journal :
The Analyst
Accession number :
edsair.doi.dedup.....00baf9283e648f5ff08d7b49012fb308