Back to Search
Start Over
A PCR-free technology to detect and quantify microRNAs directly from human plasma
- Source :
- The Analyst. 143:5676-5682
- Publication Year :
- 2018
- Publisher :
- Royal Society of Chemistry (RSC), 2018.
-
Abstract
- A novel sensitive, specific and rapid method for the detection and quantification of microRNAs without requiring extraction from their biological sources is now available using a novel chemical based, PCR-free technology for nucleic acid testing. In this study, we both demonstrate how this method can be used to profile miR-451a, an important miRNA in erythropoiesis, and compare with the gold standard RT-qPCR.
- Subjects :
- 0301 basic medicine
Detection limit
Chemistry
Nucleic Acid Hybridization
Galactosides
Computational biology
Gold standard (test)
Nucleic Acid Testing
beta-Galactosidase
Biochemistry
Analytical Chemistry
MicroRNAs
03 medical and health sciences
Spectrometry, Fluorescence
030104 developmental biology
Limit of Detection
Human plasma
Oxazines
microRNA
Biomarkers, Tumor
Electrochemistry
Humans
Environmental Chemistry
Erythropoiesis
Spectroscopy
Subjects
Details
- ISSN :
- 13645528 and 00032654
- Volume :
- 143
- Database :
- OpenAIRE
- Journal :
- The Analyst
- Accession number :
- edsair.doi.dedup.....00baf9283e648f5ff08d7b49012fb308
- Full Text :
- https://doi.org/10.1039/c8an01397g