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Proteomic analysis of the effect of triterpenes from Patrinia heterophylla on leukemia K562 cells
- Source :
- Journal of Ethnopharmacology. 144:576-583
- Publication Year :
- 2012
- Publisher :
- Elsevier BV, 2012.
-
Abstract
- For centuries, Patrinia heterophylla had been used in China to treat many diseases including tumor. Triterpenes has been identified as the major active constituents in Patrinia heterophylla. To elucidate the antitumor mechanism of triterpenes from Patrinia heterophylla1 (TPH), a proteomic analysis is carried out with TPH treatment in K562 cells. The total proteins extracted from TPH treated K562 cells are analyzed by two dimensional gel electrophoresis (2-DE) and compared with those untreated K562 cells. Mass spectrometry is applied to identify the differentially expressed proteins. Twenty-three differentially expressed significant proteins are discovered. Eight proteins are later identified by mass spectrometry (MALDI-TOF-MS) and Mascot software. Among them, four proteins are up-regulated (Aldolase A, Glyceraldehyde-3-phosphate dehydrogenase, Flavin reductase and Hemoglobin subunit) and four proteins were down-regulated (Heat-shock protein 90 〈Alpha〉 (HSP90-〈Alpha〉), Eukaryotic translation initiation factor 5A, Moesin, tublin) by TPH treatment in K562 cells. The identified proteins are associated with energy metabolism, oxidative stress, apoptosis, signal transduction, differential induction, and protein biosynthesis. These findings might provide valuable insights into the antitumor mechanism of TPH in K562 cells.
- Subjects :
- Proteomics
Protein subunit
Antineoplastic Agents
Patrinia
Peptide Initiation Factors
Tubulin
Drug Discovery
Protein biosynthesis
Humans
Electrophoresis, Gel, Two-Dimensional
HSP90 Heat-Shock Proteins
Medicine, Chinese Traditional
Pharmacology
Leukemia
Two-dimensional gel electrophoresis
biology
Microfilament Proteins
Aldolase A
RNA-Binding Proteins
biology.organism_classification
Molecular biology
Triterpenes
Hemoglobin Subunits
Biochemistry
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
biology.protein
Signal transduction
K562 Cells
K562 cells
Subjects
Details
- ISSN :
- 03788741
- Volume :
- 144
- Database :
- OpenAIRE
- Journal :
- Journal of Ethnopharmacology
- Accession number :
- edsair.doi.dedup.....00b5da448c5dc2d78c73b9d5b5620111