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Single-cell visualization indicates direct role of sponge host in uptake of dissolved organic matter
- Source :
- Proceedings of the Royal Society B-Biological Sciences, 286(1916):20192153. Royal Society of London, Proc Biol Sci
- Publication Year :
- 2019
-
Abstract
- Marine sponges are set to become more abundant in many near-future oligotrophic environments, where they play crucial roles in nutrient cycling. Of high importance is their mass turnover of dissolved organic matter (DOM), a heterogeneous mixture that constitutes the largest fraction of organic matter in the ocean and is recycled primarily by bacterial mediation. Little is known, however, about the mechanism that enables sponges to incorporate large quantities of DOM in their nutrition, unlike most other invertebrates. Here, we examine the cellular capacity for direct processing of DOM, and the fate of the processed matter, inside a dinoflagellate-hosting bioeroding sponge that is prominent on Indo-Pacific coral reefs. Integrating transmission electron microscopy with nanoscale secondary ion mass spectrometry, we track 15 N- and 13 C-enriched DOM over time at the individual cell level of an intact sponge holobiont. We show initial high enrichment in the filter-feeding cells of the sponge, providing visual evidence of their capacity to process DOM through pinocytosis without mediation of resident bacteria. Subsequent enrichment of the endosymbiotic dinoflagellates also suggests sharing of host nitrogenous wastes. Our results shed light on the physiological mechanism behind the ecologically important ability of sponges to cycle DOM via the recently described sponge loop.
- Subjects :
- Nutrient cycle
Nitrogen
General Biochemistry, Genetics and Molecular Biology
Dissolved organic carbon
Animals
Organic matter
Symbiosis
General Environmental Science
Invertebrate
chemistry.chemical_classification
geography
geography.geographical_feature_category
General Immunology and Microbiology
biology
Ecology
Coral Reefs
General Medicine
Coral reef
biology.organism_classification
Porifera
Holobiont
Sponge
chemistry
Environmental chemistry
Dinoflagellida
General Agricultural and Biological Sciences
Nanoscale secondary ion mass spectrometry
Subjects
Details
- Language :
- English
- ISSN :
- 09628452
- Volume :
- 286
- Issue :
- 1916
- Database :
- OpenAIRE
- Journal :
- Proceedings of the Royal Society B-Biological Sciences
- Accession number :
- edsair.doi.dedup.....00955b38b9163af8698730d2c3761aff