Back to Search
Start Over
TP53 and p16INK4A, but not H-KI-Ras, are involved in tumorigenesis and progression of pleomorphic adenomas
- Publication Year :
- 2006
-
Abstract
- The putative role of TP53 and p16INK4A tumor suppressor genes and Ras oncogenes in the development and progression of salivary gland neoplasias was studied in 28 cases of pleomorphic adenomas (PA), 4 cases of cystic adenocarcinomas, and 1 case of carcinoma ex-PA. Genetic and epigenetic alterations in the above genes were analyzed by Polymerase Chain Reaction/Single Strand Conformational Polymorphism (PCR/SSCP) and sequencing and by Methylation Specific-PCR (MS-PCR). Mutations in TP53 were found in 14% (4/28) of PAs and in 60% (3/5) of carcinomas. Mutations in H-Ras and K-Ras were identified in4%(1/28) and7% (2/28) of PAs, respectively. Only 20% (1/5) of carcinomas screened displayed mutations in K-Ras. p16INK4A promoter hypermethylation was found in 14% (4/28) of PAs and 100% (5/5) carcinomas. All genetic and epigenetic alterations were detected exclusively in the epithelial and transitional tumor components, and were absent in the mesenchymal parts. Our analysis suggests that TP53 mutations and p16INK4A promoter methylation, but not alterations in the H-Ras and K-Ras genes, might be involved in the malignant progression of PA into carcinoma. J. Cell. Physiol. 207: 654–659, 2006. 2006 Wiley-Liss, Inc.
- Subjects :
- Adenoma
Genotype
Physiology
Clinical Biochemistry
Biology
medicine.disease_cause
Methylation
Epigenesis, Genetic
Proto-Oncogene Proteins p21(ras)
medicine
Carcinoma
Humans
Epigenetics
TP53
Gene
Cyclin-Dependent Kinase Inhibitor p16
Base Sequence
Single-strand conformation polymorphism
Cell Biology
medicine.disease
Molecular biology
Cell Transformation, Neoplastic
Mutation
Disease Progression
Tumor Suppressor Protein p53
Carcinogenesis
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....008f93fdb661236823f6def408f4be01