MOESM3 of Musashi2 contributes to the maintenance of CD44v6+ liver cancer stem cells via notch1 signaling pathway

Additional file 3: Figure S5 A. Western blot showed that the expression of Numb had no significant difference when MSI2 was down-regulated in CD44v6+ cells or up-regulated in CD44v6- cells. B. Significantly differential expression genes (fold change ≥2, p≤0.05) between MSI2 shRNA 1 group and control group. Blue histogram represented down-regulated genes and the red represented up-regulated genes in the MSI2 shRNA 1 group compared to the control group. C. Western blot showed that overexpression of LFNG in CD44v6- HCC cells increased the expression of key components of Notch1 pathway (including Notch1, NICD, Hey1 and Hes1) but MSI2 had no significant change. β-actin was used as a normalized control. D. Western blot showed that the activation of Notch1 signaling caused by MSI2 overexpression could be inhibited by LFNG silencing in CD44v6- cells. E. LFNG protein levels in LFNG shRNA cells compared with corresponding control cells. β-actin was used as a normalized control. Figure S6 The result of positive control (SNRNP70) and negative control (U1) of RIP assays.