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Evaluation of meiotic abnormalities and pollen viability in aposporous and sexual tetraploid Paspalum notatum (Poaceae)
- Source :
- Plant Systematics and Evolution. 298:1625-1633
- Publication Year :
- 2012
- Publisher :
- Springer Science and Business Media LLC, 2012.
-
Abstract
- We analyzed anaphase I configurations and pollen viability in aposporous and sexual tetraploid (2n = 4x = 40) cytotypes of Paspalum notatum. Five natural aposporous accessions and three experimentally obtained sexual individuals were used. In addition, 16 (8aposporous and 8 sexual) F1 hybrids, previously classified by their mode of reproduction, were analyzed. Cytogenetic observations revealed normal and abnormal anaphase I configurations in both aposporous and sexual genotypes. Anaphase I abnormalities were mainly laggard chromosomes, chromatin bridges, and micronuclei. On average, 44.36 % of aposporous meiocytes and 29.66 % of sexual ones showed abnormal anaphase I configurations. The total numbers of normal and abnormal anaphase I were highly significantly different between aposporous and sexual strains. The pollen viability test indicated that aposporous individuals had significantly more non-viable pollen than sexual ones; a positive correlation (r = 0.71; r2 = 0.50) between the variables was detected. Analysis of aposporous and sexual hybrids confirmed differences in the numbers of normal and abnormal anaphase I patterns in the aposporous and sexual parents. However, similar proportions of viable pollen were produced by both groups of hybrids. In this case, the variables were not correlated (r = 0.23; r2 = 0.05). Data from this study indicated that aposporous strains had a genetic rearrangement affecting meiosis that was absent in the experimentally obtained sexual individuals and that it was transmitted to the progeny. The possible association between meiotic abnormalities and the inheritance of apospory is discussed. Fil: Podio, Maricel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Nordeste. Instituto de Botánica del Nordeste (i); Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Laboratorio de Biología Molecular; Argentina Fil: Siena, Lorena Adelina. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Laboratorio de Biología Molecular; Argentina Fil: Hojsgaard, Diego Hernan. Universitat of Gottingen; Alemania Fil: Stein, Juliana. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Laboratorio de Biología Molecular; Argentina Fil: Quarin, Camilo Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Nordeste. Instituto de Botánica del Nordeste (i); Argentina Fil: Ortiz, Juan Pablo Amelio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Nordeste. Instituto de Botánica del Nordeste (i); Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Laboratorio de Biología Molecular; Argentina
- Subjects :
- Genetics
POLLEN VIABILITY
ANAPHASE I
Plant Science
Meiocyte
Biology
medicine.disease_cause
biology.organism_classification
APOMIXIS
Meiosis
APOSPORY
CIENCIAS AGRÍCOLAS
Agronomía, reproducción y protección de plantas
Apomixis
Pollen
MEIOTIC ABNORMALITIES
Micronucleus test
medicine
Agricultura, Silvicultura y Pesca
Paspalum notatum
Ecology, Evolution, Behavior and Systematics
Hybrid
Anaphase
Subjects
Details
- ISSN :
- 16156110 and 03782697
- Volume :
- 298
- Database :
- OpenAIRE
- Journal :
- Plant Systematics and Evolution
- Accession number :
- edsair.doi.dedup.....006d4b3d6a508eb007b5f2bb8aa8f5b0