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Sequencing and de Novo Assembly of Abaca (Musa textilis Née) var. Abuab Genome
- Source :
- Genes, Volume 12, Issue 8, Genes, Vol 12, Iss 1202, p 1202 (2021)
- Publication Year :
- 2021
- Publisher :
- MDPI AG, 2021.
-
Abstract
- Abaca (Musa textilis Née), an indigenous crop to the Philippines, is known to be the source of the strongest natural fiber. Despite its huge economic contributions, research on crop improvement is limited due to the lack of genomic data. In this study, the whole genome of the abaca var. Abuab was sequenced using Illumina Novaseq 6000 and Pacific Biosciences Single-Molecule Real-Time Sequel. The genome size of Abuab was estimated to be 616 Mbp based on total k-mer number and volume peak. Its genome was assembled at 65× depth, mapping 95.28% of the estimated genome size. BUSCO analysis recovered 78.2% complete BUSCO genes. A total of 33,277 gene structures were predicted which is comparable to the number of predicted genes from recently assembled Musa spp. genomes. A total of 330 Mbp repetitive elements were also mined, accounting to 53.6% of the genome length. Here we report the sequencing and genome assembly of the abaca var. Abuab that will facilitate gene discovery for crop improvement and an indispensable source for genetic diversity studies in Musa.
- Subjects :
- 0106 biological sciences
0301 basic medicine
Manila hemp
Musa textilis Née
Sequence assembly
QH426-470
de novo assembly
Biology
01 natural sciences
Genome
Article
Crop
03 medical and health sciences
Genetics
Gene
Genome size
Genetics (clinical)
fiber crop
Whole genome sequencing
whole genome sequencing
Musa spp
Genetic diversity
Musa
biology.organism_classification
Plant Breeding
030104 developmental biology
NGS
Musa textilis
Abuab
Genome, Plant
010606 plant biology & botany
Subjects
Details
- ISSN :
- 20734425
- Volume :
- 12
- Database :
- OpenAIRE
- Journal :
- Genes
- Accession number :
- edsair.doi.dedup.....006233c93491de984824a55ef66f2d6d