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Altered Subpopulations of Red Blood Cells and Post-treatment Anemia in Malaria

Authors :
Charlotte Chambrion
Mallorie Depond
Lucia Angella
Oussama Mouri
Eric Kendjo
Aurélie Fricot-Monsinjon
Camille Roussel
Sylvestre Biligui
Ilhame Tantaoui
Aida Taieb
Nicolas Argy
Sandrine Houzé
Renaud Piarroux
Jean-Yves Siriez
Stéphane Jaureguiberry
Sébastien Larréché
Marc Théllier
Nicolas Cenac
Pierre Buffet
Papa Alioune Ndour
Biologie Intégrée du Globule Rouge (BIGR (UMR_S_1134 / U1134))
Institut National de la Transfusion Sanguine [Paris] (INTS)-Université de La Réunion (UR)-Institut National de la Santé et de la Recherche Médicale (INSERM)-CHU Pointe-à-Pitre/Abymes [Guadeloupe] -Université des Antilles (UA)-Université Paris Cité (UPCité)
Centre National de Référence du Paludisme [CHU Pitié-Salpétrière] (CNRpalu)
CHU Pitié-Salpêtrière [AP-HP]
Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)
Service d'immuno-hématologie pédiatrique [CHU Necker]
Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-CHU Necker - Enfants Malades [AP-HP]
Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)
AP-HP - Hôpital Bichat - Claude Bernard [Paris]
Hôpital Robert Debré Paris
Hôpital Robert Debré
AP-HP Hôpital Bicêtre (Le Kremlin-Bicêtre)
Hôpital d'Instruction des Armées Begin
Service de Santé des Armées
Institut de Recherche en Santé Digestive (IRSD )
Université Toulouse III - Paul Sabatier (UT3)
Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Ecole Nationale Vétérinaire de Toulouse (ENVT)
Institut National Polytechnique (Toulouse) (Toulouse INP)
Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP)
Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)
French Ministry of Research
GR-Ex laboratory of excellence
ANR-18-CE17-0018,PHeSMalEBiPPP,Anémie hémolytique post-traitement dans le paludisme grave: Elucidation de la clairance biomécanique des globules rouges pittés pour la prédiction et la prévention(2018)
SEGUIN, Nathalie
APPEL À PROJETS GÉNÉRIQUE 2018 - Anémie hémolytique post-traitement dans le paludisme grave: Elucidation de la clairance biomécanique des globules rouges pittés pour la prédiction et la prévention - - PHeSMalEBiPPP2018 - ANR-18-CE17-0018 - AAPG2018 - VALID
Source :
Frontiers in Physiology, Frontiers in Physiology, 2022, 13, 8 p. ⟨10.3389/fphys.2022.875189⟩
Publication Year :
2022
Publisher :
Frontiers Media SA, 2022.

Abstract

In acute malaria, the bulk of erythrocyte loss occurs after therapy, with a nadir of hemoglobin generally observed 3–7 days after treatment. The fine mechanisms leading to this early post-treatment anemia are still elusive. We explored pathological changes in RBC subpopulations by quantifying biochemical and mechanical alterations during severe malaria treated with artemisinin derivatives, a drug family that induce “pitting” in the spleen. In this study, the hemoglobin concentration dropped by 1.93 G/dl during therapy. During the same period, iRBC accounting for 6.12% of all RBC before therapy (BT) were replaced by pitted-RBC, accounting for 5.33% of RBC after therapy (AT). RBC loss was thus of 15.9%, of which only a minor part was due to the loss of iRBC or pitted-RBC. When comparing RBC BT and AT to normal controls, lipidomics revealed an increase in the cholesterol/phosphatidylethanolamine ratio (0.17 versus 0.24, p < 0.001) and cholesterol/phosphatidylinositol ratio (0.36 versus 0.67, p = 0.001). Using ektacytometry, we observed a reduced deformability of circulating RBC, similar BT and AT, compared to health control donors. The mean Elongation Index at 1.69Pa was 0.24 BT and 0.23 AT vs. 0.28 in controls (p < 0.0001). At 30Pa EI was 0.56 BT and 0.56 AT vs. 0.60 in controls (p < 0.001). The retention rate (rr) of RBC subpopulations in spleen-mimetic microsphere layers was higher for iRBC (rr = 20% p = 0.0033) and pitted-RBC (rr = 19%, p = 0.0031) than for healthy RBC (0.12%). Somewhat surprisingly, the post-treatment anemia in malaria results from the elimination of RBC that were never infected.

Details

Language :
English
ISSN :
1664042X
Volume :
13
Database :
OpenAIRE
Journal :
Frontiers in Physiology
Accession number :
edsair.doi.dedup.....004d9fd6715e242280af09f9207a7481
Full Text :
https://doi.org/10.3389/fphys.2022.875189